中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (28): 2199-2205.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

高同型半胱氨酸血症对子痫前期的发生有促进作用

  

  • 收稿日期:2012-05-11 修回日期:2012-08-20 出版日期:2012-10-05 发布日期:2012-10-05

Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine

Jun Wang1, Jing Ge2, Liu Yang3, Haiyan Zhang2, Xuli Li2, Dan Xue2   

  1. 1 Department of Gynaecology and Obstetrics, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China 2 Department of Gynaecology and Obstetrics, the 202 Hospital of Chinese PLA, Shenyang 110003, Liaoning Province, China 3 Shenyang Women and Children Health Center, Shenyang 110014, Liaoning Province, China
  • Received:2012-05-11 Revised:2012-08-20 Online:2012-10-05 Published:2012-10-05
  • Contact: Jun Wang, Department of Gynaecology and Obstetrics, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
  • About author:Jun Wang☆, M.D., Associate chief physician, Master’s supervisor, Department of Gynaecology and Obstetrics, General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China

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Abstract:

Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D- aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.

Key words: hyperhomocysteinemia, homocysteine, preeclampsia, frontal cortex, N-methyl-D-aspartate receptor, nerve excitability, cell apoptosis, pregnancy, brain, neural regeneration