中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (27): 2115-2122.

• 原著:退行性病与再生 • 上一篇    下一篇

止颤粉对帕金森病大鼠脑内黑质信号传导及凋亡相关基因表达的影响

  

  • 收稿日期:2012-05-26 修回日期:2012-08-15 出版日期:2012-09-25 发布日期:2012-09-25

Effects of Zhichan powder on signal transduction and apoptosis-associated gene expression in the substantia nigra of Parkinson’s disease rats

Jiajun Chen1, Jinshu Ma2, Yafei Qiu2, Shihong Yi2, Yongmao Liu2, Qingwei Zhou3, Pengguo Zhang4, Quan Wan5, Ye Kuang3   

  1. 1 Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China
    2 Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China
    3 Department of Biochemistry and Molecular Biology, Institute for Regenerative Medicine, Jilin University, Changchun 130021, Jilin Province, China
    4 Department of Image, Second Clinical Hospital, Jilin University, Changchun 130021, Jilin Province, China
    5 Department of Biotechnology, Life Science College, Jilin University, Changchun 130012, Jilin Province, China
  • Received:2012-05-26 Revised:2012-08-15 Online:2012-09-25 Published:2012-09-25
  • Contact: Yongmao Liu, M.D., Professor, Department of Immunology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, Jilin Province, China; Qingwei Zhou, M.D., Associate professor, Department of Biochemistry and Molecular Biology, Institute for Regenerative Medicine, Jilin University, Changchun 130021, Jilin Province, China yongmao@jlu.edu.cn; zhouqw@jlu.edu.cn
  • About author:Jiajun Chen, Professor, Department of Neurology, China-Japan Union Hospital, Jilin University, Changchun 130031, Jilin Province, China

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Abstract:

Previous studies have shown that Zhichan powder elevated immunity and suppressed oxidation in mice. Rat models of Parkinson’s disease were induced by stereotaxically injecting 6-hydroxydopamine into the substantia nigra. The rat models were intragastrically treated with Zhichan powder, which is composed of milkvetch root, ginseng, bunge swallowwort root, himalayan teasel root, Magnolia officinalis, Ligustrum lucidum Ait. and szechwan lovage rhizome. Immunohistochemistry and reverse transcription-PCR results demonstrated that mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cytochrome C, Bax, caspase-3, and p53 significantly increased, but Bcl-2 expression significantly decreased in the substantia nigra of rats with Parkinson’s disease. Following Zhichan powder administration, mRNA and protein expression of tumor necrosis factor receptor 1, Fas, caspase-8, cytochrome C, Bax, caspase-3, and p53 diminished, but Bcl-2 expression increased in the rat substantia nigra. These results indicate that Zhichan powder regulates signal transduction protein expression, inhibits apoptosis, and exerts therapeutic effects on Parkinson’s disease.

Key words: Zhichan powder, Parkinson’s disease, 6-hydroxydopamine, signal transduction, apoptosis, substantia nigra, traditional Chinese medicine, degenerative disease, neural regeneration