中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (3): 435-440.doi: 10.4103/1673-5374.179055

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

促进缺血再灌注脑损伤后血管生成的补阳还五汤

  

  • 收稿日期:2015-07-29 出版日期:2016-03-15 发布日期:2016-03-15
  • 基金资助:

    中国国家自然科学基金项目(81072799)

Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair

Zhen-qiang Zhang 1, Jun-ying Song 1, Ya-quan Jia 1, Yun-ke Zhang 2   

  1. 1 Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
    2 First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou, Henan Province, China
  • Received:2015-07-29 Online:2016-03-15 Published:2016-03-15
  • Contact: Yun-ke Zhang, M.D.,henanzyk@126.com.
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of China, No. 81072799.

摘要:

中药补阳还五汤经典方具有保护脑缺血再灌注损伤的作用,但其作用机制尚未完全明确。实验预先给予大鼠补阳还五汤15 mL/kg灌胃连续3d而后以应用大脑中动脉闭塞方法建立脑缺血再灌注模型。发现大鼠脑梗死体积减小,血浆中与血管发生密切相关的血管内皮生长因子和ITGαvβ3含量增加,脑组织中血管内皮生长因子和CD34表达升高,而在补阳还五汤灌胃1d后侧脑室连续7d注射血管生成抑制因子的大鼠上述现象明显减弱。说明补阳还五汤是以促进缺血再灌注脑损伤的血管生成,改善脑循环的机制达到促进脑损伤的修复效果。

关键词: 神经再生, 补阳还五汤, 脑缺血再灌注损伤, 缺血性脑血管病, 整合素αvβ3, 血管内皮生长因子, 血管生成, CD34

Abstract:

Buyanghuanwu decoction has been shown to protect against cerebral ischemia/reperfusion injury, but the underlying mechanisms remain unclear. In this study, rats were intragastrically given Buyanghuanwu decoction, 15 mL/kg, for 3 days. A rat model of cerebral ischemia/reperfusion injury was established by middle cerebral artery occlusion. In rats administered Buyanghuanwu decoction, infarct volume was reduced, serum vascular endothelial growth factor and integrin αvβ3 levels were increased, and brain tissue vascular endothelial growth factor and CD34 expression levels were increased compared with untreated animals. These effects of Buyanghuanwu decoction were partially suppressed by an angiogenesis inhibitor (administered through the lateral ventricle for 7 consecutive days). These data suggest that Buyanghuanwu decoction promotes angiogenesis, improves cerebral circulation, and enhances brain tissue repair after cerebral ischemia/reperfusion injury.

Key words: nerve regeneration, Buyanghuanwu decoction, cerebral ischemia/reperfusion injury, ischemic cerebrovascular disease, integrin αvβ3, vascular endothelial growth factor, angiogenesis, CD34, neural regeneration