中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (5): 771-778.doi: 10.4103/1673-5374.182704

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

冷诱导RNA结合蛋白对亚低温治疗外伤性脑损伤的神经保护作用

  

  • 收稿日期:2016-04-23 出版日期:2016-05-20 发布日期:2016-05-20
  • 基金资助:

    国家自然科学基金项目(81303091)

Neuroprotective effects of cold-inducible RNA-binding protein during mild hypothermia on traumatic brain injury

Guan Wang1, 2, *, Jian-ning Zhang3, Jia-kui Guo2, Ying Cai4 , Hong-sheng Sun4, Kun Dong2, Cheng-gang Wu2   

  1. "1 Postgraduate Institution, Tianjin Medical University, Tianjin, China 2 Department of Neurosurgery, Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 3 Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin, China 4 Department of Neurosurgery, Tianjin Huanhu Hospital, Tianjin, China"
  • Received:2016-04-23 Online:2016-05-20 Published:2016-05-20
  • Contact: Guan Wang, Doctoral candidate, neurocrown@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81303091.

摘要:

研究表明,冷诱导RNA结合蛋白与温度有关的调控蛋白,在亚低温时对大脑、心脏、肝脏等组织或器官发挥保护作用。实验观察(31 ± 0.5)℃亚低温治疗48 h的外伤性脑损伤大鼠发现,亚低温治疗显著抑制了脑损伤大鼠大脑皮质、海马、下丘脑中的细胞凋亡,并增加上述部位中冷诱导RNA结合蛋白mRNA及蛋白的表达,其作用在下丘脑最为显著。而使用siRNA沉默后,冷诱导RNA结合蛋白可抑制亚低温的作用。实验还发现冷诱导RNA结合蛋白表达沉默与MEK激活无关,但抑制ERK-1/2的激活。说明冷诱导RNA结合蛋白可通过影响ERK-1/2的激活,干预相关细胞凋亡,从而在亚低温治疗外伤性脑损伤中发挥了神经保护作用。 

orcid: 0000-0001-8310-7142(Guan Wang)

关键词: 神经再生, 外伤性脑损伤, 亚低温, 冷诱导RNA结合蛋白, MEK, ERK, 抗凋亡

Abstract:

"Cold-inducible RNA-binding protein (CIRP), a key regulatory protein, could be facilitated by mild hypothermia in the brain, heart and liver. This study observed the effects of mild hypothermia at 31 ± 0.5°C on traumatic brain injury in rats. Results demonstrated that mild hypothermia suppressed apoptosis in the cortex, hippocampus and hypothalamus, facilitated CIRP mRNA and protein expression in these regions, especially in the hypothalamus. The anti-apoptotic effect of mild hypothermia disappeared after CIRP silencing. There was no correlation between mitogen-activated extracellular signal-regulated kinase activation and CIRP silencing. CIRP silencing inhibited extracellular signal-regulated kinase-1/2 activation. These indicate that CIRP inhibits apoptosis by affecting extracellular signal-regulated kinase-1/2 activation, and exerts a neuroprotective effect during mild hypothermia for traumatic brain injury."

Key words: nerve regeneration, traumatic brain injury, mild hypothermia, cold-inducible RNA-binding protein, mitogen-activated extracellular signal-regulated kinase, anti-apoptosis, neural regeneration