中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (5): 787-794.doi: 10.4103/1673-5374.182706

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

下丘脑不同放电类型神经元对外侧丘系背核刺激的反应：在听觉功能声源定位中的作用

  

  • 收稿日期:2016-04-06 出版日期:2016-05-20 发布日期:2016-05-20
  • 基金资助:

    国家自然科学基金项目（81271090）

Responses from two firing patterns in inferior colliculus neurons to stimulation of the lateral lemniscus dorsal nucleus

Xiao-ting Li1, Ning-yu Wang1, *, Yan-jun Wang1, Zhi-qing Xu2, Jin-feng Liu1, Yun-fei Bai2, Jin-sheng Dai1, Jing-yi Zhao1   

  1. "1 Department of Otorhinolaryngology Head and Neck Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China 2 Department of Neurophysiology, Capital Medical University, Beijing, China"
  • Received:2016-04-06 Online:2016-05-20 Published:2016-05-20
  • Contact: Ning-yu Wang, M.D., wny1128@hotmail.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81271090.

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摘要：

下丘脑中的γ-氨基丁酸能神经元被分为不同的放电类型，这种分类方法被认为与下丘脑神经元的生理功能有关。为明确每种放电类型神经元在听觉处理中确切的生理功能，我们在离体条件下对下丘脑神经元的放电特性进行研究。使用全细胞记录模式下的膜片钳技术记录其对电刺激外侧丘系背核时的放电反应。在下丘脑中一共记录到7个神经元，分属于2种类型，持续规律放电型神经元（n=3）和持续适应放电型神经元(n=4)。大部分下丘脑神经元对于电刺激外侧丘系背核以及给予荷包牡丹碱表现为极小的放电变化，但其中1个持续适应放电型神经元表现为受电刺激后外侧丘系背核压制，应用γ-氨基丁酸受体抑制剂荷包牡丹碱后恢复至原有放电水平；1个持续规律放电型神经元亦表现为受电刺激压制，但并不受荷包牡丹碱影响。结果提示下丘脑中的γ-氨基丁酸能神经元表现为持续规律放电型及持续适应放电型，γ-氨基丁酸能神经元可能表现为后者。目前，由外侧丘系背核投射至下丘脑的γ-氨基丁酸能纤维被认为与声源定位有关，不同类型神经元的放电反应可能与其在声源定位中的生理功能有关，认识其功能，希望为能解决临床听力障碍确定治疗的靶标途径。

orcid: 0000-0002-3080-2093(Ning-yu Wang)

关键词: 神经再生, 下丘脑, GABA能神经元, 放电模式, 持续规律放电型神经元, 持续适应放电型神经元, 优先效应, 持久抑制作用, 外侧丘系背核, 抑制投射

Abstract:

"The γ-aminobutyric acid neurons (GABAergic neurons) in the inferior colliculus are classified into various patterns based on their intrinsic electrical properties to a constant current injection. Although this classification is associated with physiological function, the exact role for neurons with various firing patterns in acoustic processing remains poorly understood. In the present study, we analyzed characteristics of inferior colliculus neurons in vitro, and recorded responses to stimulation of the dorsal nucleus of the lateral lemniscus using the wholecell patch clamp technique. Seven inferior colliculus neurons were tested and were classified into two firing patterns: sustained-regular (n = 4) and sustained-adapting firing patterns (n = 3). The majority of inferior colliculus neurons exhibited slight changes in response to stimulation and bicuculline. The responses of one neuron with a sustained-adapting firing pattern were suppressed after stimulation, but recovered to normal levels following application of the γ-aminobutyric acid receptor antagonist. One neuron with a sustained-regular pattern showed suppressed stimulation responses, which were not affected by bicuculline. Results suggest that GABAergic neurons in the inferior colliculus exhibit sustained-regular or sustained-adapting firing patterns. Additionally, GABAergic projections from the dorsal nucleus of the lateral lemniscus to the inferior colliculus are associated with sound localization. The different neuronal responses of various firing patterns suggest a role in sound localization. A better understanding of these mechanisms and functions will provide better clinical treatment paradigms for hearing deficiencies."

Key words: nerve regeneration, inferior colliculus, GABAergic neuron, firing pattern, sustained-regular firing pattern, sustained-adapting firing pattern, precedence effect, long-lasting inhibition, dorsal nucleus of the lateral lemniscus, inhibitory projection, neural regeneration