中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (9): 1506-1511.doi: 10.4103/1673-5374.191227

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

周围神经损伤再生过程中微管相关蛋白tau的改变

  

  • 收稿日期:2016-09-14 出版日期:2016-09-30 发布日期:2016-09-30
  • 基金资助:
    中国国家自然科学基金项目（81130080, 31300942）；973项目（2014CB542202）；江苏省自然科学基金项目（BK20150409）；江苏省高校自然科学面上项目 （15KJB180013）；南通大学科研基金项目（14R29）；南通市自然科学基金项目（MS12015043）；江苏高校优势学科项目

Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration

Guang-bin Zha, Mi Shen, Xiao-song Gu, Sheng Yi*   

  1. Jiangsu Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China
  • Received:2016-09-14 Online:2016-09-30 Published:2016-09-30
  • Contact: Sheng Yi, Ph.D., syi@ntu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81130080, 31300942; the National Key Basic Research Program of China (973 Program), No. 2014CB542202; the Natural Science Foundation of Jiangsu Province, China, No. BK20150409; the Natural Science Foundation of Jiangsu Higher Education Institutions of China, No. 15KJB180013; the Scientific Research Foundation of Nantong University of China, No. 14R29; the Natural Science Foundation of Nantong City in China, No. MS12015043; the Priority Academic Program Development of Jiangsu Higher Education Institutions of China.

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摘要：

tau是微管相关蛋白的主要组成成分，能够促进微管组装/拆卸，维持微管结构稳定且参与多种神经退行性疾病的发病，但tau蛋白在周围神经损伤后的作用目前尚无研究。为此，实验收集了坐骨神经挤压损伤模型大鼠损伤后0，1，4，7，14d时的坐骨神经，检测编码tau蛋白mRNA水平及tau蛋白的表达水平，并分析tau蛋白在损伤坐骨神经中的定位变化。PCR结果显示，与未损伤时相比，神经损伤后，编码tau和tau微管蛋白激酶1的mRNA表达均明显下降；且Western blot结果也显示，Ser 404位点磷酸化tau和总tau蛋白在神经损伤后1d时降低，而后逐渐增加；而免疫荧光染色结果显示tau蛋白定位是不变的。实验结果表明坐骨神经挤压损伤后tau蛋白的表达模式发生改变，说明其参与了周围神经修复的过程。 

orcid: 0000-0003-1316-3370 (Sheng Yi)

关键词: 神经再生, 坐骨神经损伤, 微管相关蛋白, tau, 磷酸化tau(Ser 404), tau蛋白异常磷酸化, tau微管蛋白激酶1, 微管结构, 微管组装/拆卸, 周围神经系统

Abstract: Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, whether tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/ threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in peripheral nerve repair and regeneration.

Key words: nerve regeneration, sciatic nerve crush, microtubule-associated protein, tau, phosphorylated tau (Ser 404), tau hyper-phosphorylation, tau tubulin kinase 1, microtubule structure, microtubule assembly and disassembly, peripheral nervous system, neural regeneration