中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (9): 1472-1478.doi: 10.4103/1673-5374.215259

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

细胞外信号调节激酶、P物质和神经激肽1参与隔药灸镇痛机制

  

  • 收稿日期:2017-06-29 出版日期:2017-09-15 发布日期:2017-09-15
  • 基金资助:

    中国国家自然科学基金项目（81273843, 81674073）; 国家重点基础研究发展计划（973计划）（2015CB554501）; 上海市卫计委计划项目（20144Y0153, 2017BR047）

Extracellular signal-regulated kinase, substance P and neurokinin-1 are involved in the analgesic mechanism of herb-partitioned moxibustion

Zhi-yuan Li1, 2, Yan-ting Yang1, Jue Hong3, Dan Zhang1, 3, Xiao-fei Huang1, Li-jie Wu1, Huan-gan Wu1, 3, Zheng Shi3, Jie Liu3, Yi Zhu3, Xiao-peng Ma1, 3   

  1. 1 Shanghai University of Traditional Chinese Medicine, Shanghai, China
    2 Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang Province, China
    3 Shanghai Research Institute of Acupuncture and Meridian, Shanghai, China
  • Received:2017-06-29 Online:2017-09-15 Published:2017-09-15
  • Contact: Xiao-peng Ma, Ph.D. or Dan Zhang, Ph.D.,pengpengma@163.com or zhangdan_982@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81273843, 81674073; a grant from the National Key Basic Research Program of China (973 Program), No. 2015CB554501; the Project Fund of Shanghai Municipal Commission of Health and Family Planning of China, No. 20144Y0153, 2017BR047.

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摘要：

临床上应用隔药灸可有效减轻炎症性肠病引起的内脏痛，但其镇痛机制仍无共识。有研究发现，细胞外信号调节激酶、P物质和神经激肽1能共同引起疼痛信号的转导，因此作者推测隔药灸镇痛效应可能与上述因子有关。为验证此假设，实验设计以5%三硝基苯磺酸和50%乙醇混合溶液灌肠建立炎症性肠病内脏疼痛大鼠模型，在双侧天枢（ST25）和气海（CV6）进行隔药灸约10min，1次/d，连续7d。结果显示，隔药灸能明显减轻大鼠的内脏疼痛，并降低背根神经节中细胞外信号调节激酶、P物质和神经激肽1蛋白及mRNA的表达，说明隔药灸抑制细胞外信号调节激酶、P物质和神经激肽1表达，是其发挥肠性镇痛作用的分子机制之一。

orcid:0000-0002-0582-3562(Xiao-peng Ma)
0000-0003-1388-4047(Yan-ting Yang)

关键词: 神经再生, 炎症性肠病, 内脏疼痛, 隔药灸, 镇痛, 天枢, 气海, 背根神经节, 细胞外信号调节激酶, P物质, 神经激肽1

Abstract:

Herb-partitioned moxibustion can effectively mitigate visceral pain, a major symptom in inflammatory bowel disease, but the analgesic mechanism is still unclear. Moreover, extracellular signal-regulated kinase, substance P, and neurokinin-1 are involved in formation of central hyperalgesia. Thus, we postulated that the analgesic effect of herb-partitioned moxibustion may be associated with these factors.Accordingly, in this study, we established an inflammatory bowel disease visceral pain model in rat by enema with a mixed solution of 5% trinitrobenzenesulfonic acid and 50% ethanol. Bilateral Tianshu (ST25) and Qihai (CV6) points were selected for herb-partitioned moxibustion.Our results showed that herb-partitioned moxibustion improved visceral pain and down-regulated extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA expression in dorsal root ganglia. These results indicate that down-regulation of extracellular signal-regulated kinase, substance P, and neurokinin-1 protein and mRNA may be a central mechanism for the analgesic effect of herb-partitioned moxibustion.

Key words: nerve regeneration, inflammatory bowel disease, visceral pain, herb-partitioned moxibustion, analgesic effect, Tianshu (ST25), Qihai (CV6), dorsal root ganglion, extracellular signal-regulated kinase, substance P, neurokinin-1, neural regeneration