中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (22): 1688-1694.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

神经干细胞/祖细胞体外分化过程中伴随成纤维细胞生长因子8和Sonic Hedgehog信号通路的动态表达和分泌变化

  

  • 收稿日期:2012-02-14 修回日期:2012-05-03 出版日期:2012-08-05 发布日期:2012-08-05

Changes in expression and secretion patterns of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway molecules during murine neural stem/progenitor cell differentiation in vitro

Jiang Lu1, 2, Kehuan Lu1, Dongsheng Li3   

  1. 1 State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China
    2 Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China
    3 Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China
  • Received:2012-02-14 Revised:2012-05-03 Online:2012-08-05 Published:2012-08-05
  • Contact: Kehuan Lu, M.D., Professor, Doctoral supervisor, State Key Laboratory for Conser-vation and Utilization of Subtropical Agro- biore-sources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China; Dongsheng Li, M.D., Professor, Hubei Key Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China khlu@gxu.edu.cn;dsli@yymc.edu.cn
  • About author:Jiang Lu☆, Studying for doctorate, Lecturer, State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China; Department of Anesthesiology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei Province, China

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Abstract:

In the present study, we investigated the dynamic expression of fibroblast growth factor 8 and Sonic Hedgehog signaling pathway related factors in the process of in vitro hippocampal neural stem/progenitor cell differentiation from embryonic Sprague-Dawley rats or embryonic Kunming species mice, using fluorescent quantitative reverse transcription-PCR and western blot analyses. Results demonstrated that the dynamic expression of fibroblast growth factor 8 was similar to fi-broblast growth factor receptor 1 expression but not to other fibroblast growth factor receptors. Enzyme-linked immunosorbent assay demonstrated that fibroblast growth factor 8 and Sonic Hedgehog signaling pathway protein factors were secreted by neural cells into the intercellular niche. Our experimental findings indicate that fibroblast growth factor 8 and Sonic Hedgehog ex-pression may be related to the differentiation of neural stem/progenitor cells.