中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (22): 1695-1702.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

杆状病毒转导抗肌萎缩蛋白基因及抗肌萎缩蛋白相关蛋白双敲除小鼠脂肪干细胞的转基因表达和分化

  

  • 收稿日期:2012-04-28 修回日期:2012-07-27 出版日期:2012-08-05 发布日期:2012-08-05

Transgene expression and differentiation of baculovirus-transduced adipose-derived stem  cells from dystrophin-utrophin double knock-out mouse

Qiuling Li1, 2, Qiongxiang Zhai2, Jia Geng3, Hui Zheng4, Fei Chen5, Jie Kong1, Cheng Zhang1   

  1. 1 Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
    2 Department of Pediatrics, Guangdong General Hospital, Guangdong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China
    3 Department of Neurology, First Affiliated Hospital, Kunming Medical College, Kunming 650032, Yunnan Province, China
    4 Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
    5 Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou 510085, Guangdong Province, China
  • Received:2012-04-28 Revised:2012-07-27 Online:2012-08-05 Published:2012-08-05
  • Contact: Cheng Zhang, M.D., Professor, Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China chengzhang100@hotmail. com
  • About author:Qiuling Li☆, M.D., Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China; Department of Pediatrics, Guangdong General Hospital, Guang-dong Neuroscience Institute, Guangdong Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China

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Abstract:

In this study, recombinant baculovirus carrying the microdystrophin and β-catenin genes was used to infect adipose-derived stem cells from a dystrophin-utrophin double knock-out mouse. Results showed that, after baculovirus transgene infection, microdystrophin and β-catenin genes were effectively expressed in adipose-derived stem cells from the dystrophin-utrophin double knock-out mouse. Furthermore, this transgenic expression promoted adipose-derived stem cell differentiation into muscle cells, but inhibited adipogenic differentiation. In addition, protein expression related to the microdystrophin and Wnt/β-catenin signaling pathway was upregulated. Our experimental findings indicate that baculovirus can successfully deliver the microdystrophin and β-catenin genes into adipose-derived stem cells, and the microdystrophin and Wnt/β-catenin signaling pathway plays an important role in myogenesis of adipose-derived stem cells in the dystrophin-utrophin double knock-out mouse.

Key words: baculovirus, adipose-derived stem cells, Duchenne muscular dystrophy, microdystrophin, β-catenin, myogenesis, gene therapy, neural regeneration