中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (22): 1703-1708.

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

音猥因子可提高神经干细胞N-myc基因的表

  

  • 收稿日期:2012-03-21 修回日期:2012-06-30 出版日期:2012-08-05 发布日期:2012-08-05

Sonic hedgehog elevates N-myc gene expression in neural stem cells

Dongsheng Liu1, Shouyu Wang1, Yan Cui1, Lun Shen1, Yanping Du1, Guilin Li2, Bo Zhang1, Renzhi Wang2   

  1.  
    1 Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China
    2 Chinese Academy of Medical Sciences, Department of Neurosurgery, Peking Union Medical College Hospital, Beijing 100730, China
  • Received:2012-03-21 Revised:2012-06-30 Online:2012-08-05 Published:2012-08-05
  • Contact: Bo Zhang, M.D., Professor, Chief physician, Department of Neurosurgery, First Affi-liated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China zhangbodl@126.com
  • About author:Dongsheng Liu★, Master, Department of Neurosurgery, First Affiliated Hospital, Dalian Medical University, Dalian 116011, Liaoning Province, China

PDF

1097

Abstract:

Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gli1 and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

Key words: stem cells, neural stem cells, sonic hedgehog signal pathway, N-myc gene, proliferation, target gene, neural regeneration