中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (8): 1789-1801.doi: 10.4103/1673-5374.389304

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

外侧隔核向海马腹侧CA1区的投射如何影响社交能力

  

  • 出版日期:2024-08-15 发布日期:2024-01-03
  • 基金资助:
    国家自然科学基金项目(82171521);山东省泰山学者专项资助项目(tsqn202211368);山东省自然科学基金项目(ZR2022YQ65,ZR2021MH073,ZR2019PH109);山东省医疗卫生技术发展计划项目(202003090720,202003070728,2019WS329),滨州医科大学科研基金项目(BY2018KJ21)

How do lateral septum projections to the ventral CA1 influence sociability?

Dan Wang1, 2, 3, 4, #, Di Zhao1, 2, 3, 4, #, Wentao Wang1, 2, 3, 4, Fengai Hu1, 2, 3, 4, Minghu Cui2, 3, 4, Jing Liu1, 2, 3, 4, Fantao Meng1, 2, 3, 4, Cuilan Liu1, 2, 3, 4, Changyun Qiu1, 2, 3, 4, Dunjiang Liu1, 2, 3, 4, Zhicheng Xu1, 2, 3, 4, Yameng Wang1, 2, 3, 4, Yu Zhang2, 5, *, Wei Li1, 2, 3, *, Chen Li1, 2, 3, 4, *#br#   

  1. 1Department of Rehabilitation Medicine, Binzhou Medical University Hospital, Binzhou, Shandong Province, China; 2Medical Research Center, Binzhou Medical University Hospital, Binzhou, Shandong Province, China; 3Institute for Metabolic & Neuropsychiatric Disorders, Binzhou Medical University Hospital, Binzhou, Shandong Province, China; 4Department of Psychology, Binzhou Medical University Hospital, Binzhou, Shandong Province, China; 5College of Nursing, Binzhou Medical University, Binzhou, Shandong Province, China
  • Online:2024-08-15 Published:2024-01-03
  • Contact: Chen Li, PhD, lc_0625@163.com; Wei Li, PhD, yishengliwei@163.com; Yu Zhang, PhD, zhangy0605@126.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 82171521 (to CL); the Special Funds of Taishan Scholars Project of Shandong Province, No. tsqn202211368 (to CL); the Natural Science Foundation of Shandong Province, Nos. ZR2022YQ65 (to CL), ZR2021MH073 (to CL), and ZR2019PH109 (to WW); the Projects of Medical and Health Technology Development Program in Shandong Province, China, Nos. 202003090720 (to DZ), 202003070728 (to JL), and 2019WS329 (to DW), and the Scientific Research Foundation of Binzhou Medical University, No. BY2018KJ21 (to DW).

摘要:

社会功能障碍是多种神经精神疾病的危险因素。既往研究显示外侧隔核相关通路在介导社会行为中起着至关重要的作用,然而,外侧隔核及相关脑区之间的连接在社会行为方面的确切功能作用尚不清楚。此次实验在小鼠中使用电生理和化学遗传学方法进行了三箱实验,以确定外侧隔核向海马腹侧CA1区的投射如何影响社交能力。结果表明,γ-氨基丁酸能神经元可在社交经历后被激活,而化学遗传学激活外侧隔核中γ-氨基丁酸能神经元能增强社交行为,而外侧隔核中γ-氨基丁酸能神经元具有海马腹侧CA1区的正向神经纤维投射,并与海马腹侧CA1区中γ-氨基丁酸能和谷氨酸能神经元均形成突触连接,但是仅特异性调节海马腹侧CA1区中谷氨酸能神经元的活性。特异性阻断海马腹侧CA1区中γ-氨基丁酸A受体能弱化特异性激活外侧隔核中γ-氨基丁酸能神经元对社交行为的促进作用。这些发现支持了外侧隔核γ-氨基丁酸能神经元向海马腹侧CA1谷氨酸能神经元的神经环路对小鼠社交偏好和社交新奇性的调控作用,提示此神经环路或核团或可成为社交障碍及其相关疾病干预治疗的新靶点。

https://orcid.org/0000-0001-5385-6249 (Chen Li); https://orcid.org/0000-0002-6378-7723 (Wei Li); https://orcid.org/0000-0002-9959-005X (Yu Zhang)

关键词: 外侧隔核, 海马腹侧CA1区, GABA能神经元, 谷氨酸能神经元, 神经投射, 神经兴奋性, GABA受体, 化学遗传学, 社会偏好, 社交新奇性

Abstract: Social dysfunction is a risk factor for several neuropsychiatric illnesses. Previous studies have shown that the lateral septum (LS)-related pathway plays a critical role in mediating social behaviors. However, the role of the connections between the LS and its downstream brain regions in social behaviors remains unclear. In this study, we conducted a three-chamber test using electrophysiological and chemogenetic approaches in mice to determine how LS projections to ventral CA1 (vCA1) influence sociability. Our results showed that gamma-aminobutyric acid (GABA)-ergic neurons were activated following social experience, and that social behaviors were enhanced by chemogenetic modulation of these neurons. Moreover, LS GABAergic neurons extended their functional neural connections via vCA1 glutamatergic pyramidal neurons, and regulating LSGABA→vCA1Glu neural projections affected social behaviors, which were impeded by suppressing LS-projecting vCA1 neuronal activity or inhibiting GABAA receptors in vCA1. These findings support the hypothesis that LS inputs to the vCA1 can control social preferences and social novelty behaviors. These findings provide new insights regarding the neural circuits that regulate sociability.

Key words: chemogenetics, GABA receptor, GABAergic neurons, glutamatergic neurons, lateral septum, neural excitability, neural projection, social novelty, social preference, ventral CA1