中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (3): 536-540.doi: 10.4103/1673-5374.228759

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

内质网应激传感器老星型胶质细胞特异诱导物质有助于脊髓损伤后的星形胶质细胞增生

  

  • 收稿日期:2018-01-16 出版日期:2018-03-15 发布日期:2018-03-15

Endoplasmic reticulum stress transducer old astrocyte specifically induced substance contributes to astrogliosis after spinal cord injury

Atsushi Takazawa1, Naosuke Kamei1, 2, Nobuo Adachi1, Mitsuo Ochi1   

  1. 1 Department of Orthopaedic Surgery, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan
    2 Medical Center for Translational & Clinical Research, Hiroshima University Hospital, Hiroshima, Japan
  • Received:2018-01-16 Online:2018-03-15 Published:2018-03-15
  • Contact: Naosuke Kamei, M.D., Ph.D.,nahkamei@hiroshima-u.ac.jp.
  • Supported by:

    This study was supported by MEXT/JSPS KAKENHI Grant-in-Aid for Scientific Research (C) to NK (Grant No. 17K10931).

摘要:

orcid:0000-0001-5076-0145(Naosuke Kamei)

Abstract:

Old astrocyte specifically induced substance (OASIS) is an endoplasmic reticulum (ER) stress transducer specifically expressed in astrocytes and osteoblasts. OASIS regulates the differentiation of neural precursor cells into astrocytes in the central nervous system. This study aimed to elucidate the involvement of ER stress responses stimulated via OASIS in astrogliosis following spinal cord injury. In a mouse model of spinal cord contusion injury, OASIS mRNA and protein expression were evaluated at days 7 and 14. A significant increase in OASIS mRNA on day 7 and an increase in protein on days 7 and 14 was observed in injured spinal cords. Immunostaining on day 7 revealed co-localization of OASIS and astrocytes in the periphery of the injury site. Furthermore, anti-OASIS small interfering RNA (siRNA) was injected at the injury sites on day 5 to elucidate the function of OASIS. Treatment with anti-OASIS siRNA caused a significant decrease in OASIS mRNA on day 7 and protein on days 7 and 14, and was associated with the inhibition of astrogliosis and hindlimb motor function recovery. Results of our study show that OASIS expression synchronizes with astrogliosis and is functionally associated with astrogliosis after spinal cord injury.

Key words: unfolded protein response, cAMP-response element binding protein/activating transcription factor protein family, C57BL/6, contusion injury, reactive astrocyte, functional recovery, real-time polymerase chain reaction, western blot, immunohistochemistry, glial fibrillary acidic protein