中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (8): 1440-1446.doi: 10.4103/1673-5374.235301

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

石墨烯纳米带TexasPEG可促进腰髓横断大鼠运动功能的恢复

  

  • 收稿日期:2018-05-31 出版日期:2018-08-15 发布日期:2018-08-15
  • 基金资助:

    韩国政府资助的国家研究基金会（NRF）资助

Effect of Graphene Nanoribbons (TexasPEG) on locomotor function recovery in a rat model of lumbar spinal cord transection

C-Yoon Kim1, 2, William K. A. Sikkema3, Jin Kim2, Jeong Ah Kim4, James Walter5, Raymond Dieter5, Hyung-Min Chung1, Andrea Mana6, James M. Tour3, Sergio Canavero6   

  1. 1 Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, Korea
    2 Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Korea
    3 Department of Chemistry, Department of Materials Science and NanoEngineering, and The NanoCarbon Center, Rice University, Houston, TX, USA
    4 Biomedical Omics Group, Korea Basic Science Institute, Cheongju-si, Chungbuk, Korea
    5 Research Service, Hines Veterans Administration Hospital, Hines, IL, USA
    6 HEAVEN/GEMINI International Collaborative Group, Turin, Italy
  • Received:2018-05-31 Online:2018-08-15 Published:2018-08-15
  • Contact: C-Yoon Kim, V.M.D. or Sergio Canavero, M.D.,vivavets@gmail.com or sercan@inwind.it.
  • Supported by:

    This research was supported by a grant from the National Research Foundation (NRF) funded by the Korean government (NRF-2015M3A9C7030091 and NRF-2015R1C1A1A02037047).

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摘要：

已有研究显示，膜促融合剂聚乙二醇可促进横断脊髓的融合。为评估膜促融合剂聚乙二醇化石墨烯纳米带（在PEG600中以1wt％分散体制备时称为“TexasPEG”）对脊髓横断面腰段水平（L1）的模型大鼠运动功能恢复及细胞水平变化的影响。实验分为两部分进行：（1）体内实验：将大鼠L1水平脊髓完全横断，然后在损伤部位应用TexasPEG，5周后以Basso Beattie Bresnahan（BBB）评分进行运动功能评估，并在损伤脊髓段进行星胶质细胞标志物胶质纤维酸性蛋白和神经元标志物神经丝200免疫组化染色。（1）体外实验：以倒置显微镜观察TexasPEG作用72h对SH-SY5Y细胞神经突生长的影响。 两部分实验均结果显示，TexasPEG减弱了胶质瘢痕的形成，促进了神经突的再生，从而使脊髓横断模型大鼠运动功能得以恢复。

 

orcid:0000-0003-1199-8024(C-Yoon Kim)

关键词: 神经再生, 脊髓横断, 脊髓融合, GEMINI, TexasPEG, 石墨烯纳米带

Abstract:

A sharply transected spinal cord has been shown to be fused under the accelerating influence of membrane fusogens such as polyethylene glycol (PEG) (GEMINI protocol). Previous work provided evidence that this is in fact possible. Other fusogens might improve current results. In this study, we aimed to assess the effects of PEGylated graphene nanoribons (PEG-GNR, and called “TexasPEG” when prepared as 1wt% dispersion in PEG600) versus placebo (saline) on locomotor function recovery and cellular level in a rat model of spinal cord transection at lumbar segment 1 (L1) level. In vivo and in vitro experiments (n = 10 per experiment) were designed. In the in vivo experiment, all rats were submitted to full spinal cord transection at L1 level. Five weeks later, behavioral assessment was performed using the Basso Beattie Bresnahan (BBB) locomotor rating scale. Immunohistochemical staining with neuron marker neurofilament 200 (NF200) antibody and astrocytic scar marker glial fibrillary acidic protein (GFAP) was also performed in the injured spinal cord. In the in vitro experiment, the effects of TexasPEG application for 72 hours on the neurite outgrowth of SH-SY5Y cells were observed under the inverted microscope. Results of both in vivo and in vitro experiments suggest that TexasPEG reduces the formation of glial scars, promotes the regeneration of neurites, and thereby contributes to the recovery of locomotor function of a rat model of spinal cord transfection.

Key words: nerve regeneration, spinal cord transfection, spinal cord fusion, GEMINI, TexasPEG, graphene nanoribbons