中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (4): 692-698.doi: 10.4103/1673-5374.247473

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

几种不同神经转位修复大鼠长段尺神经缺损

  

  • 出版日期:2019-04-15 发布日期:2019-04-15
  • 基金资助:

    国家自然科学基金（31571236，31571235）; 中国国家重点研究发展计划（2016YFC1101604）; 中国国家重点基础研究计划（973计划）（2014CB542200）; 中国教育部创新计划编号IRT_16R01; 北京科技新星交叉项目（2018019）

Repair of long segmental ulnar nerve defects in rats by several different kinds of nerve transposition

Fei Yu 1 , You-Lai Yu 2, Su-Ping Niu 1 , Pei-Xun Zhang 1 , Xiao-Feng Yin 1 , Na Han 1 , Ya-Jun Zhang 1 , Dian-Ying Zhang 1 , Yu-Hui Kou 1, Bao-Guo Jiang 1   

  1. 1 Peking University People’s Hospital, Beijing, China
    2 The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China
  • Online:2019-04-15 Published:2019-04-15
  • Contact: Yu-Hui Kou, MD, PhD, yuhuikou@bjmu.edu.cn; Bao-Guo Jiang, MD, PhD, jiangbaoguo@vip.sina.com.
  • Supported by:

    This research was continuously funded by the National Natural Science Foundation of China, No. 31571236, 31571235 (to YHK, PXZ); the National Key Research and Development Program of China, No. 2016YFC1101604 (to DYZ); the National Key Basic Research Program of China (973 Program), No. 2014CB542200 (to BGJ); the Ministry of Education Innovation Program of China, No. IRT_16R01 (to BGJ); the Beijing Science and Technology New Star Cross Program of China, No. 2018019 (to PXZ).

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摘要：

作者以往研究发现周围神经损伤修复过程中存在轴芽倍数再生现象，证实了损伤周围神经存在一定的修复潜能，因此提出了系统的神经转位修复技术治疗严重的周围神经损伤，并证实在神经转位修复过程中，脊髓前角运动神经元再生的神经纤维能够有效长入修复的远端神经及靶肌肉组织中，利于恢复运动功能。为探讨不同神经转位修复长段周围神经缺损后的有效再生及神经功能恢复的促进作用，实验以肌皮神经、胸内侧神经、桡神经肌支和骨间前神经（旋前方肌肌支）转位修复SD大鼠长段（2mm）的尺神经缺损，以尺神经原位修复为对照。3个月后，以神经电生理刺激，锇酸染色和HE染色分别对大鼠屈腕功能、神经再生和神经支配肌肉恢复情况进行了评估。结果提示不同的供体神经转位修复长段尺神经缺损，可使相应神经的脊髓前角运动神经元的细胞体实现轴突功能的再支配，一定程度上恢复患肢的功能。

orcid: 0000-0001-8436-5266(Bao-Guo Jiang)

关键词: 神经转位修复, 锥形套管, 小间隙套接缝合, 尺神经, 靶器官, 肌肉, 神经再支配, 神经再生

Abstract:

Multiple regeneration of axonal buds has been shown to exist during the repair of peripheral nerve injury, which confirms a certain repair potential of the injured peripheral nerve. Therefore, a systematic nerve transposition repair technique has been proposed to treat severe peripheral nerve injury. During nerve transposition repair, the regenerated nerve fibers of motor neurons in the anterior horn of the spinal cord can effectively grow into the repaired distal nerve and target muscle tissues, which is conducive to the recovery of motor function. The aim of this study was to explore regeneration and nerve functional recovery after repairing a long-segment peripheral nerve defect by transposition of different donor nerves. A long-segment (2 mm) ulnar nerve defect in Sprague-Dawley rats was repaired by transposition of the musculocutaneous nerve, medial pectoral nerve, muscular branches of the radial nerve and anterior interosseous nerve (pronator quadratus muscle branch). In situ repair of the ulnar nerve was considered as a control. Three months later, wrist flexion function, nerve regeneration and innervation muscle recovery in rats were assessed using neuroelectrophysiological testing, osmic acid staining and hematoxylin-eosin staining, respectively. Our findings indicate that repair of a long-segment ulnar nerve defect with different donor nerve transpositions can reinnervate axonal function of motor neurons in the anterior horn of spinal cord and restore the function of affected limbs to a certain extent.

Key words: nerve regeneration, nerve transposition repair, conical sleeve, small gap sleeve bridging, ulnar nerve, target organ, muscle, nerve reinnervation, neural regeneration