中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (13): 1013-1019.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Survivin-specific small interfering RNAs enhance sensitivity of glioma U-87MG cells to paclitaxel by promoting apoptosis

  

  • 收稿日期:2011-12-07 修回日期:2012-04-16 出版日期:2012-05-05 发布日期:2012-05-05

Survivin-specific small interfering RNAs enhance sensitivity of glioma U-87MG cells to paclitaxel by promoting apoptosis

Yunliang Xie1, 2, Yanbo Liu2, Weigao Shen2, Bo Zhang2, Qun Liu1   

  1. 1  Department of Neurology, First Clinical Hospital of Bethune Medical College of Jilin University, Changchun 130001, Jilin Province, China
    2  Department of Anatomy, Basic Medical College, Beihua University, Jilin 132013, Jilin Province, China
  • Received:2011-12-07 Revised:2012-04-16 Online:2012-05-05 Published:2012-05-05
  • Contact: Qun Liu, Ph.D., Professor,Department of Neurology,First Clinical Hospital of Bethune Medical College of Jilin University, Changchun 130001, Jilin Province,China; Weigao Shen, Ph.D.,Professor, Department of Anatomy, Basic Medical College, Beihua University,Jilin 132013, Jilin Province,China shenweigao@163.com
  • About author:Yunliang Xie☆, Studying for doctorate, Attending physician, Department of Neurology, First Clinical Hospital of Bethune Medical College of Jilin University,Changchun 130001, Jilin Province, China; Department of Anatomy, Basic Medical College, Beihua University,Jilin 132013, Jilin Province,China Yunliang Xie and Yanbo Liu contributed equally to this article.

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Abstract:

A survivin siRNA expression vector was transfected into glioma U-87MG cells and these cells were then treated with paclitaxel. The results showed that survivin-specific siRNA combined with paclitaxel treatment synergistically inhibited glioma U-87MG cell proliferation and promoted apoptosis. This treatment also inhibited the expression of the cell cycle regulatory proteins, survivin,cyclinD1, c-Myc and CDK4 and enhanced the sensitivity of U-87MG cells to paclitaxel.

Key words: RNA interference, paclitaxel, survivin, glioma, neural regeneration