中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (11): 2162-2168.doi: 10.4103/1673-5374.282260

• 原著:周围神经损伤修复保护与再生 • 上一篇    

白云母纳米颗粒可减轻神经性疼痛

  


  • 出版日期:2020-11-15 发布日期:2020-08-23
  • 基金资助:

    这项研究得到了由韩国政府国家研究基金（NRF-2017R1A2B4009963）和韩国东方医学研究所基金（K18182）资助

Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord

Ju-Young Oh 1, 2, 3 , Tae-Yeon Hwang 1, 2, 3 , Jae-Hwan Jang 1, 2, 3 , Ji-Yeun Park 1, 4 , Yeonhee Ryu 5 , HyeJung Lee 1, 2 , Hi-Joon Park 1, 2, 3   

  1. 1 Acupuncture and Meridian Science Research Center, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul, Republic of Korea
    2 Department of Korean Medical Science, Graduate School of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea
    3 BK21 PLUS Korean Medicine Science Center, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea
    4 College of Korean Medicine, Daejeon University, Daejeon, Republic of Korea
    5 Korean Institute of Oriental Medicine, Daejeon, Republic of Korea
  • Online:2020-11-15 Published:2020-08-23
  • Contact: Hi-Joon Park, MD, PhD,acufind@khu.ac.kr.
  • Supported by:
    This study was supported by grants from the National Research Foundation of Korea funded by the Korean government (NRF-
    2017R1A2B4009963; to JYO, TYH, JHJ and HJP) and from the Korea Institute of Oriental Medicine (grant K18182; to JYP, YR and HJP).

PDF

151

摘要：

白云母是一种铝硅酸盐矿物，已在传统医学中用作抗炎药，但其对神经性疼痛的功效尚未得到研究。实验旨在评估白云母纳米颗粒是否可通过控制炎症过程，以减轻神经性疼痛症状。实验以部分坐骨神经结扎的方法建立神经性疼痛小鼠模型，给予该模型以肌肉注射白云母纳米颗粒（4 mg/100μL）以评估其对神经性疼痛的影响。结果显示，白云母纳米颗粒可显著减轻部分坐骨神经结扎引起的机械性和冷性异常性疼痛，对脊髓星形胶质细胞和小胶质细胞活化具有抑制作用，并可降低脊髓中促炎细胞因子白细胞介素1β、肿瘤坏死因子α、白细胞介素6和单核细胞趋化蛋白1的表达，并抑制坐骨神经中神经损伤标志活化转录因子3的表达。这些结果表明，白云母纳米颗粒对PSNL诱导的神经性疼痛的镇痛作用可能是由于抑制星形胶质细胞和小胶质细胞以及促炎细胞因子的激活所致。说明白云母纳米颗粒有潜力用于神经性疼痛的治疗。

orcid: 0000-0002-0538-2664 (Hi-Joon Park) 

         0000-0001-9141-0133 (Ju-Young Oh)

关键词: 星形胶质细胞, 白云母, 纳米粒子, 神经性疼痛, 坐骨神经部分结扎, 药物针刺, 促炎细胞因子, 小胶质细胞, 脊髓

Abstract: Despite numerous efforts to overcome neuropathic pain, various pharmacological drugs often fail to meet the needs and have many side effects. Muscovite is an aluminosilicate mineral that has been reported to have an anti-inflammatory effect, but the efficacy of muscovite for neuropathic pain has not been investi- gated. Here, we assessed whether muscovite nanoparticles can reduce the symptoms of pain by controlling the inflammatory process observed in neuropathic pain. The analgesic effects of muscovite nanoparticles were explored using partial sciatic nerve ligation model of neuropathic pain, in which one-third to one- half of the nerve trifurcation of the sciatic nerve was tightly tied to the dorsal side. Muscovite nanoparticles (4 mg/100 μL) was given intramuscularly to evaluate its effects on neuropathic pain (3 days per week for 4 weeks). The results showed that the muscovite nanoparticle injections significantly alleviated partial sci- atic nerve ligation-induced mechanical and cold allodynia. In the spinal cord, the muscovite nanoparticle injections exhibited inhibitory effects on astrocyte and microglia activation and reduced the expression of pro-inflammatory cytokines, such as interleukin-1β, tumor necrosis factor-α, interleiukin-6 and monocyte chemoattractant protein-1, which were upregulated in the partial sciatic nerve ligation model. Moreover, the muscovite nanoparticle injections resulted in a decrease in activating transcription factor 3, a neuronal injury marker, in the sciatic nerve. These results suggest that the analgesic effects of muscovite nanoparticle on partial sciatic nerve ligation-induced neuropathic pain may result from inhibiting activation of astro- cytes and microglia as well as pro-inflammatory cytokines. We propose that muscovite nanoparticle is a potential anti-nociceptive candidate for neuropathic pain. All experimental protocols in this study were approved by the Institutional Animal Ethics Committee (IACUC) at Dongguk University, South Korea (ap- proval No. 2017-022-1) on September 28, 2017.

Key words: astrocyte, microglia, muscovite, nanoparticle, neuropathic pain, partial sciatic nerve ligation, pharmacopuncture, pro-inflammatory cytokine, spinal cord