中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (9): 669-674.

• 原著:视神经损伤修复保护与再生 • 上一篇    下一篇

Puerarin antagonizes peroxyntrite-induced injury in retinal pigment epithelial cells

  

  • 收稿日期:2011-12-24 修回日期:2012-02-22 出版日期:2012-03-25 发布日期:2012-03-25

Puerarin antagonizes peroxyntrite-induced injury in retinal pigment epithelial cells

Lina Hao1, Xudong Zhang2, Tao Yang3, Junling Ma1   

  1. 1  Ophthalmology Department, Hebei Province People’s Hospital, Shijiazhuang 050051, Hebei Province, China
    2  Pharmaceutical Department, Hebei Province People’s Hospital, Shijiazhuang 050051, Hebei Province, China
    3  Internal Department, the First Hospital, Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • Received:2011-12-24 Revised:2012-02-22 Online:2012-03-25 Published:2012-03-25
  • Contact: Lina Hao, Ophthalmology Department of Hebei Province People’s Hospital, Shijiazhuang 050051, Hebei Province, China jhaolina@yahoo.com.cn
  • About author:Lina Hao, M.D., Chief physician, Professor, Ophthalmology Department of Hebei Province People’s Hospital, Shijiazhuang 050051, Hebei Province, China

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Abstract:

A rat model of diabetes mellitus was established by intraperitoneal injection of streptozotocin. Three days later, the rats were intraperitoneally administered 140 mg puerarin/kg daily, for a total of 60 successive days. DNA ladder results showed increased apoptosis over time in retinal pigment epithelial cells from rats with streptozotocin-induced diabetes mellitus. Western blot analysis, Reverse transcription-PCR, immunohistochemistry, and flow cytometry results showed increased expression of 3-nitrotyrosine, a peroxyntrite marker, as well as inducible nitric synthase and Fas/FasL, in retinal pigment epithelial cells. Puerarin reversed these changes, and results demonstrated that puerarin inhibited Fas/FasL expression and alleviated peroxyntrite injury to retinal pigment epithelial cells. These results suggested that puerarin inhibited production of inducible nitric oxide synthase and directly antagonized peroxyntrite injury in retinal pigment epithelial cells.

Key words: apoptosis, Fas/FasL, inducible nitric oxide synthase, oxidative stress, puerarin, retinal pigment epithelial cells