中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (12): 2376-2382.doi: 10.4103/1673-5374.313018

• 综述:脑损伤修复保护与再生 • 上一篇    下一篇

急性硬脑膜下血肿猪模型中硫化氢和催产素系统脑沟底部定位

  

  • 出版日期:2021-12-15 发布日期:2021-05-13

Localization of the hydrogen sulfide and oxytocin systems at the depth of the sulci in a porcine model of acute subdural hematoma

Oscar McCook1, *, Angelika Scheuerle2, Nicole Denoix1, 3, Thomas Kapapa4, Peter Radermacher1, Tamara Merz1    

  1. 1Institute for Anesthesiological Pathophysiology and Process Engineering, Ulm University Medical Center, Ulm, Germany; 2Department of Neuropathology, Ulm University Medical Center, Günzburg, Germany; 3Clinic for Psychosomatic Medicine and Psychotherapy, Ulm University Medical Center, Ulm, Germany; 4Department of Neurosurgery, Ulm University Medical Center, Ulm, Germany
  • Online:2021-12-15 Published:2021-05-13
  • Contact: Oscar McCook, Oscar.mccook@uni-ulm.de.
  • Supported by:
    This work was supported by a grant from the Deutsche Bundeswehr and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - Projektnummer 251293561 - SFB 1149 (to PR); a grant from the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) - Projektnummer 251293561 - SFB 1149 and Ulm University – Baustein-Programm (to TM).

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摘要：

Neural Regen Res：压力诱导脑损伤猪模型可能是研究人脑损伤的适用模型

来自德国乌尔姆大学的Oscar McCook团队最近在猪急性硬膜下血肿(ASDH)诱导的脑损伤临床前模型中进行了连续双侧多模式脑监测、脑靶向重症监护以及对ASDH损伤的脑免疫组化表征。他们发现急性硬膜下血肿是由左顶叶硬膜下注入自体血液引起的，这导致了脑内压力的短暂升高，血肿诱导的脑损伤与同侧脑白蛋白外溢、氧化应激、反应性星形胶质细胞增生和小胶质细胞活化有关。McCook团队发现大脑内催产素、催产素受体、胱硫氨酸-γ-裂解酶和胱硫氨酸-β-合成酶的表达模式特别有趣：这四种蛋白都共定位于脑沟底部，压力诱发的脑损伤会激发最大应激力。在这种情况下，与啮齿类动物的大脑相比，猪是一个与人体非常相关的转化模型。猪的大脑结构与人类非常相似：存在脑回和脑沟(回脑)、白质与灰质的比例和小脑幕。McCook团队在猪模型中观察到与人类已知的损伤相同的空间关系，这表明此模型可能是研究人脑损伤和测试新治疗方法有效性的适用模型。

文章在《中国神经再生研究（英文版）》杂志2021年12月12期发表。

https://orcid.org/0000-0001-7879-7695 (Oscar McCook)

Abstract: In the porcine model discussed in this review, the acute subdural hematoma was induced by subdural injection of autologous blood over the left parietal cortex, which led to a transient elevation of the intracerebral pressure, measured by bilateral neuromonitoring. The hematoma-induced brain injury was associated with albumin extravasation, oxidative stress, reactive astrogliosis and microglial activation in the ipsilateral hemisphere. Further proteins and injury markers were validated to be used for immunohistochemistry of porcine brain tissue. The cerebral expression patterns of oxytocin, oxytocin receptor, cystathionine-γ-lyase and cystathionine-β-synthase were particularly interesting: these four proteins all co-localized at the base of the sulci, where pressure-induced brain injury elicits maximum stress. In this context, the pig is a very relevant translational model in contrast to the rodent brain. The structure of the porcine brain is very similar to the human: the presence of gyri and sulci (gyrencephalic brain), white matter to grey matter proportion and tentorium cerebelli. Thus, pressure-induced injury in the porcine brain, unlike in the rodent brain, is reflective of the human pathophysiology. 

Key words: animal modeling, brain edema, cystathionine-β-synthase, cystathionine-γ-lyase, gyrencephalic brain, immunohistochemistry, intensive care unit, large animal model, neuromonitoring, oxytocin receptor