中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (4): 256-260.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats

  

  • 收稿日期:2011-09-15 修回日期:2012-01-05 出版日期:2012-02-05 发布日期:2012-02-05

Dispase rapidly and effectively purifies Schwann cells from newborn mice and adult rats

Jiaxue Zhu1, 2, Jinbao Qin1, 2, Zunli Shen1, 2, James D. Kretlow3, Xiaopan Wang1, 2, Zhangyin Liu1, 2, Yuqing Jin1, 2   

  1. 1 Department of Plastic and Reconstructive Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
    2 Stem Cell and Tissue Engineering Research Center, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China
    3 Department of Bioengineering, Rice University, Houston 77251-1892, TX, USA
  • Received:2011-09-15 Revised:2012-01-05 Online:2012-02-05 Published:2012-02-05
  • Contact: Zunli Shen, M.D., Ph.D., Chief physician, Department of Plastic and Reconstructive Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; Stem Cell and Tissue Engineering Research Center, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; Yuqing Jin, M.D., Ph.D., Assistant researcher, Department of Plastic and Reconstructive Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; Stem Cell and Tissue Engineering Research Center, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China zunli_shen@yahoo.com.cn yuqing.jin@gmail.com
  • About author:Jiaxue Zhu☆, M.D., Department of Plastic and Reconstructive Surgery, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China; Stem Cell and Tissue Engineering Research Center, Shanghai First People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China Jiaxue Zhu and Jinbao Qin contributed equally to this paper.

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Abstract:

In the present study, Schwann cells were isolated from the sciatic nerve of neonatal mice and purified using dispase and collagenase. Results showed that after the first round of purification with dispase, most of the Schwann cells appeared round in shape and floated in culture solution after 15 minutes. In addition, cell yield and cell purity were higher when compared to the collagenase group. After the second round of purification, the final cell yield for the dispase group was higher than that for the collagenase group, but no significant difference was found in cell purity. Moreover, similar results in cell quantity and purity were observed in adult Sprague-Dawley rats. These findings indicate that purification with dispase can result in the rapid isolation of Schwann cells with a high yield and purity.

Key words: dispase, differential detachment, purification, Schwann cell, neural regeneration