中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (4): 261-267.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells Superior effects over original formula of Buyang Huanwu decoction

  

  • 收稿日期:2011-09-23 修回日期:2011-12-25 出版日期:2012-02-05 发布日期:2012-02-05

The active principle region of Buyang Huanwu decoction induced differentiation of bone marrow-derived mesenchymal stem cells into neural-like cells Superior effects over original formula of Buyang Huanwu decoction

Jinghui Zheng1, Yi Wan2, Jianhuai Chi2, Dekai Shen2, Tingting Wu2, Weimin Li2, Pengcheng Du2   

  1. 1 Grade Three Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine of China, Ruikang Hospital, Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonomous Region, China
    2 Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
  • Received:2011-09-23 Revised:2011-12-25 Online:2012-02-05 Published:2012-02-05
  • Contact: Yi Wan, Master, Associate professor, Anhui University of Traditional Chinese Medi-cine, Hefei 230038, Anhui Province, China wanyidoctor@hotmail.com
  • About author:Jinghui Zheng☆, M.D., Grade Three Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine of China, Ruikang Hospital, Guangxi Traditional Chinese Medical University, Nanning 530011, Guangxi Zhuang Autonom-ous Region, China

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Abstract:

The present study induced in vitro-cultured passage 4 bone marrow-derived mesenchymal stem cells to differentiate into neural-like cells with a mixture of alkaloid, polysaccharide, aglycone, glycoside, essential oils, and effective components of Buyang Huanwu decoction (active principle region of decoction for invigorating yang for recuperation). After 28 days, nestin and neuron-specific enolase were expressed in the cytoplasm. Reverse transcription-PCR and western blot analyses showed that nestin and neuron-specific enolase mRNA and protein expression was greater in the active principle region group compared with the original formula group. Results demonstrated that the active principle region of Buyang Huanwu decoction induced greater differentiation of rat bone marrow-derived mesenchymal stem cells into neural-like cells in vitro than the original Buyang Huanwu decoction formula.

Key words: active principle region, bone marrow-derived mesenchymal stem cells, Buyang Huanwu decoction, differentiation, nerve cells