中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (11): 2526-2529.doi: 10.4103/1673-5374.339003

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

针刺可激活大脑躯体感觉皮质中的神经元和星形胶质细胞

  

  • 出版日期:2022-11-15 发布日期:2022-04-23
  • 基金资助:
    国家重点研发计划项目（2016YFC1306702）；国家自然科学基金项目（81771455）；广东省科技计划项目（2018B030334001）；广东省自然科学基金项目（2019A1515011772）

In vivo neuronal and astrocytic activation in somatosensory cortex by acupuncture stimuli

Xiao-Yue Chang1, 2, #, Kai Chen1, #, Tong Cheng1, #, Pui To Lai2, Li Zhang1, 3, 4, *, Kwok-Fai So1, 2, 3, 4, 5, *, Edward S. Yang2, *   

  1. 1Key Laboratory of CNS Regeneration (Minstry of Education), Guangdong-Hong Kong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou, Guangdong Province, China; 2Department of Electrical and Electronic Engineering, The University of Hong Kong, Hong Kong Special Administrative Region, China; 3Center for Brain Science and Brain-Inspired Intelligence, Guangdong-Hong Kong-Macao Greater Bay Area, Guangzhou, Guangdong Province, China; 4Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Guangzhou, Guangdong Province, China; 5State Key Laboratory of Brain and Cognitive Science, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong Special Administrative Region, China
  • Online:2022-11-15 Published:2022-04-23
  • Contact: Li Zhang, PhD, zhangli@jnu.edu.cn; Kwok-Fai So, PhD, hrmaskf@hku.hk; Edward S. Yang, PhD, esyang123@hotmail.com.
  • Supported by:
    This study was supported by National Key Research and Development Program of China, No. 2016YFC1306702 (to KFS and LZ); the National Natural Science Foundation of China, No. 81771455 (to KFS); Science and Technology Program of Guangdong Province of China, No. 2018B030334001 (to KFS); and the Natural Science Foundation of Guangdong of China, No. 2019A1515011772 (to LZ).

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摘要：

针灸是一种在中国广为流传的疗法，已有三千多年的历史，但对其神经机制的探索仍不完整。作者假设针刺刺激下神经元和星形胶质细胞在可独立和协作地作用，为验证这一观点，实验使用双光子活体钙成像技术对经外周穴位ST36(足三里)手针刺激的小鼠进行观察，发现其初级体感皮质中锥体神经元和星形胶质细胞的钙信号增强，且刺激可导致星形胶质细胞迟发性钙瞬变。进一步利用化学遗传方法抑制锥体神经元后，可见星形胶质细胞的活动增强。表明针刺可激活躯体感觉皮质神经元和星形胶质细胞的活动，这也为神经元和星形胶质细胞参与针刺治疗提供了证据。

https://orcid.org/0000-0003-1489-0879 (Li Zhang); https://orcid.org/0000-0003-4039-4246 (Kwok-Fai So)

关键词: 针刺, 躯体感觉皮质, 神经元, 星形胶质细胞, 化学遗传学, N-甲基-D-天冬氨酸受体, 瞬时受体电位通道蛋白A1, 双光子活体钙成像技术

Abstract: Acupuncture is a medical treatment that has been widely practiced in China for over 3000 years, yet the neural mechanisms of acupuncture are not fully understood. We hypothesized that neurons and astrocytes act independently and synergistically under acupuncture stimulation. To investigate this, we used two-photon in vivo calcium recording to observe the effects of acupuncture stimulation at ST36 (Zusanli) in mice. Acupuncture stimulation in peripheral acupoints potentiated calcium signals of pyramidal neurons and astrocytes in the somatosensory cortex and resulted in late-onset calcium transients in astrocytes. Chemogenetic inhibition of neurons augmented the astrocytic activity. These findings suggest that acupuncture activates neuronal and astrocytic activity in the somatosensory cortex and provide evidence for the involvement of both neurons and astrocytes in acupuncture treatment.

Key words: acupuncture, astrocyte, chemogenetic, neuron, N-methyl-D-aspartate receptor, somatosensory cortex, transient receptor potential A1, two-photon in vivo imaging