中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2012, Vol. 7 ›› Issue (1): 6-12.

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

Functional proteomics of adenosine triphosphatase system in the rat striatum during aging

  

  • 收稿日期:2011-10-25 修回日期:2011-12-05 出版日期:2012-01-05 发布日期:2012-01-05

Functional proteomics of adenosine triphosphatase system in the rat striatum during aging

Roberto Federico Villa, Federica Ferrari, Antonella Gorini   

  1. Department of Forensic Medicine and Pharmacological-Toxicological Sciences, Division of Pharmacological and Toxicological Sciences, Laboratory of Pharmacology and Molecular Medicine of Central Nervous System, University of Pavia, Via Ferrata, 9-27100 Pavia, Ital
  • Received:2011-10-25 Revised:2011-12-05 Online:2012-01-05 Published:2012-01-05
  • Contact: Ro-berto Federico Villa, De-partment of Forensic Medi-cine and Pharmacological- Toxicological Sciences, Division of Pharmacological and Toxicological Sciences, Laboratory of Pharmacology and Molecular Medicine of Central Nervous System, University of Pavia, Via Ferrata, 9-27100 Pavia, Italy robertofederico.villa@unipv.it
  • About author:Roberto Federico Villa☆, D.Sc., M.D., Ph.D., Associate professor, Department of Forensic Medicine and Pharmacological- Toxicolog-ical Sciences, Division of Pharmacological and Tox-icological Sciences, Labora-tory of Pharmacology and Molecular Medicine of Cen-tral Nervous System, Univer-sity of Pavia, Via Ferrata, 9-27100 Pavia, Italy

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Abstract:

The maximum rates of adenosine triphosphatase (ATPase) systems related to energy consumption were systematically evaluated in synaptic plasma membranes isolated from the striata of male Wistar rats aged 2, 6, 12, 18, and 24 months, because of their key role in presynaptic nerve ending homeostasis. The following enzyme activities were evaluated: sodium-potassium-magnesium adenosine triphosphatase (Na+, K+, Mg2+-ATPase); ouabain-insensitive magnesium adenosine triphosphatase (Mg2+-ATPase); sodium-potassium adenosine triphosphatase (Na+, K+-ATPase); direct magnesium adenosine triphosphatase (Mg2+-ATPase); calcium-magnesium adenosine triphosphatase (Ca2+, Mg2+-ATPase); and acetylcholinesterase. The results showed that Na+, K+-ATPase decreased at 18 and 24 months, Ca2+, Mg2+-ATPase and acetylcholinesterase decreased from 6 months, while Mg2+-ATPase was unmodified. Therefore, ATPases vary independently during aging, suggesting that the ATPase enzyme systems are of neuropathological and pharmacological importance. This could be considered as an experimental model to study regeneration processes, because of the age-dependent modifications of specific synaptic plasma membranes. ATPases cause selective changes in some cerebral functions, especially bioenergetic systems. This could be of physiopathological significance, particularly in many central nervous system diseases, where, during regenerative processes, energy availability is essential.

Key words: ATPase, synaptic plasma membranes, aging, striatum, functional proteomics