中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (1): 84-91.doi: 10.4103/1673-5374.125334

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针联合脐带血间充质干细胞移植治疗脑缺血再灌注损伤

  

  • 收稿日期:2013-11-25 出版日期:2014-01-05 发布日期:2014-01-05

Electro-acupuncture at Conception and Governor vessels and transplantation of umbilical cord blood-derived mesenchymal stem cells for treating cerebral ischemia/reperfusion injury

Haibo Yu1, Pengdian Chen1, Zhuoxin Yang2, Wenshu Luo1, Min Pi1, Yonggang Wu1, Ling Wang1   

  1. 1 Affiliated Shenzhen Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province, China
    2 Affiliated Shenzhen Maternity & Child Healthcare Hospital, Southern Medical University, Shenzhen, Guangdong Province, China
  • Received:2013-11-25 Online:2014-01-05 Published:2014-01-05
  • Contact: Zhuoxin Yang, M.D., Affiliated Shenzhen Maternity & Child Healthcare Hospital, Southern Medical University, Shenzhen 518028, Guangdong Province, China, dalexisu@126.com.

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摘要：

间充质干细胞可移植治疗脑缺血再灌注损伤，促进血管新生和神经功能恢复；长期针刺任督脉治疗脑缺血再灌注损伤也能达到较好的治疗效果。基于此，我们假设电针任督脉和间充质干细胞移植联合治疗脑缺血再灌注，在促进血管新生和神经功能恢复方面的疗效更佳。故将人脐带血间充质干细胞注射至脑缺血再灌注24 h后的大鼠脑内纹状体和皮质区域，并在移植后24 h给予任督脉电针干预。改良神经功能缺损评分及免疫组织化学染色结果显示，电针任督脉和人脐血间充质干细胞移植干预的脑缺血再灌注大鼠神经功能缺损明显改善，脑梗死边缘区血管内皮生长因子免疫阳性细胞数量明显增加，效果均优于单独人脐带血间充质干细胞移植治疗。结果说明，电针任督脉联合人脐带血间充质干细胞移植促进脑缺血再灌注大鼠神经功能恢复及血管新生的效果好，二者具有协同作用。

关键词: 神经再生, 针灸, 人脐血间充质干细胞, 电针, 脑缺血再灌注, 血管内皮生长因子, 血管新生, 任脉, 督脉, 神经功能缺损评分

Abstract:

Mesenchymal stem cell transplantation is a novel means of treating cerebral ischemia/reperfusion, and can promote angiogenesis and neurological functional recovery. Acupuncture at Conception and Governor vessels also has positive effects as a treatment for cerebral ischemia/reperfusion. Therefore, we hypothesized that electro-acupuncture at Conception and Governor vessels plus mesenchymal stem cell transplantation may have better therapeutic effects on the promotion of angiogenesis and recovery of neurological function than either treatment alone. In the present study, human umbilical cord blood-derived mesenchymal stem cells were isolated, cultured, identified and intracranially transplanted into the striatum and subcortex of rats at     24 hours following cerebral ischemia/reperfusion. Subsequently, rats were electro-acupunctured at Conception and Governor vessels at 24 hours after transplantation. Modified neurological severity scores and immunohistochemistry findings revealed that the combined interventions of electro-acupuncture and mesenchymal stem cell transplantation clearly improved neurological impairment and up-regulated vascular endothelial growth factor expression around the ischemic focus. The combined intervention provided a better outcome than mesenchymal stem cell transplantation alone. These findings demonstrate that electro-acupuncture at Conception and Governor vessels and mesenchymal stem cell transplantation have synergetic effects on promoting neurological function recovery and angiogenesis in rats after cerebral ischemia/reperfusion.

Key words: nerve regeneration, acupuncture, human umbilical cord blood-derived mesenchymal stem cells, electro-acupuncture, cerebral ischemia/reperfusion, vascular endothelial growth factor, angiogenesis, Conception vessel, Governor vessel, modified neurological severity score, NSFC grant, neural regeneration