中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (3): 260-267.doi: 10.4103/1673-5374.128218

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

当归多糖可以减轻氧化应激导致的神经细胞损伤

  

  • 收稿日期:2014-01-20 出版日期:2014-02-15 发布日期:2014-02-15

Polysaccharides from Angelica sinensis alleviate neuronal cell injury caused by oxidative stress

Tao Lei1, Haifeng Li2, Zhen Fang1, Junbin Lin1, Shanshan Wang1, Lingyun Xiao2, Fan Yang2, Xin Liu2, Junjian Zhang1, Zebo Huang2, Weijing Liao1   

  1. 1 Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan
    University, Wuhan, Hubei Province, China
    2 School of Pharmaceutical Sciences, Wuhan University, Wuhan, Hubei Province, China
  • Received:2014-01-20 Online:2014-02-15 Published:2014-02-15
  • Contact: Weijing Liao, M.D., Ph.D., Department of Rehabilitation Medicine, Zhongnan Hospital and Cerebral Vascular Diseases Research Center, Zhongnan Hospital, Wuhan University, 169# Donghu Road, Wuhan 430071, Hubei Province, China, weijingliao@sina.com.

摘要:

来自中国的传统中药当归具有抗氧化和神经保护作用,作者希望验证这一作用来自当归的主要有效成分当归多糖。为此,预实验首先利用氧化氢暴露损伤的PC12细胞,证实当归多糖干预后不仅能够减轻氧化应激导致的细胞毒性,减少细胞凋亡,而且能减少细胞内活性氧,并增加线粒体膜电位。进一步以局灶性脑缺血大鼠模型证实,当归多糖能提高脑缺血大鼠大脑皮质神经元的抗氧化损伤能力,增加脑缺血大鼠脑组织微血管数量,改善脑血流。说明当归多糖能够对抗氧化应激致神经细胞损伤,同时展现出减缓神经元损伤的有效性。

Abstract:

Angelica sinensis has antioxidative and neuroprotective effects. In the present study, we aimed to determine the neuroprotective effect of polysaccharides isolated from Angelica sinensis. In a preliminary experiment, Angelica sinensis polysaccharides not only protected PC12 neuronal cells from H2O2-induced cytotoxicity, but also reduced apoptosis and intracellular reactive oxygen species levels, and increased the mitochondrial membrane potential induced by H2O2 treatment. In a rat model of local cerebral ischemia, we further demonstrated that Angelica sinensis polysaccharides enhanced the antioxidant activity in cerebral cortical neurons, increased the number of microvessels, and improved blood flow after ischemia. Our findings highlight the protective role of polysaccharides isolated from Angelica sinensis against nerve cell injury and impairment caused by oxidative stress.

Key words: nerve regeneration, cerebral ischemia, Angelica sinensis, polysaccharides, antioxidation, reactive oxygen species, mitochondrial membrane potential, apoptosis, microvessels, NSFC grant, neural regeneration