中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (3): 308-317.doi: 10.4103/1673-5374.128229

• 原著:退行性病与再生 • 上一篇    下一篇

成年大鼠膈斜角带复合体区巢蛋白阳性神经元的特殊功能

  

  • 收稿日期:2013-11-25 出版日期:2014-02-15 发布日期:2014-02-15
  • 基金资助:

    广东省自然科学基金项目(S2011040004372),中央高校基本科研业务费专项资金(11ykpy05)

Special function of nestin+ neurons in the medial septum-diagonal band of Broca in adult rats

Yuhong Zhao, Kaihua Guo, Dongpei Li, Qunfang Yuan, Zhibin Yao   

  1. Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong Province, China
  • Received:2013-11-25 Online:2014-02-15 Published:2014-02-15
  • Contact: Zhibin Yao, M.D., Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China, yao.zb@163.com.
  • Supported by:

    This study was supported by the Guangdong Natural Science Foundation of China, No. S2011040004372; the Fundamental Research Funds for the Central Universities, No. 11ykpy05.

摘要:

作者前期研究发现成年大鼠膈斜角带复合体区存在稳定表达胆碱乙酰化酶的巢蛋白阳性神经元。实验通过对成熟大鼠膈斜角带复合体区巢蛋白阳性神经元向嗅球的投射及嗅神经切断后这类神经元恢复过程中变化的分析,发现这类巢蛋白阳性神经元均表达胆碱乙酰化酶,且53.63%向嗅球投射的胆碱能神经元可表达巢蛋白;而当嗅神经切断后,膈斜角带复合体各区巢蛋白阳性神经元丢失较多,但在嗅神经切断早期,这类神经元在损伤较轻的内侧隔阂及斜角带区出现明显的代偿反应,在损伤后数量减少至最低的时间明显迟于巢蛋白阴性/胆碱乙酰转移酶阳性神经元。表明这类神经元对损伤刺激的耐受力似乎比不表达巢蛋白的胆碱能神经元要强,与嗅球的纤维投射联系更密切一些,且这类神经元在损伤恢复中表现的与巢蛋白阴性/胆碱乙酰转移酶阳性神经元的差异性值得进一步深入研究。

关键词: 神经再生, 巢蛋白, 胆碱乙酰化酶, 神经递质, 膈斜角带复合体, 嗅球, 嗅神经切断, 基金资助文章

Abstract:

Nestin+ neurons have been shown to express choline acetyltransferase (ChAT) in the medial septum-diagonal band of Broca in adult rats. This study explored the projection of nestin+ neurons to the olfactory bulb and the time course of nestin+ neurons in the medial septum-diagonal band of Broca in adult rats during injury recovery after olfactory nerve transection. This study observed that all nestin+ neurons were double-labeled with ChAT in the medial septum-diagonal band of Broca. Approximately 53.6% of nestin+ neurons were projected to the olfactory bulb and co-labeled with fast blue. A large number of nestin+ neurons were not present in each region of the medial septum-diagonal band of Broca. Nestin+ neurons in the medial septum and vertical limb of the diagonal band of Broca showed obvious compensatory function. The number of nestin+ neurons decreased to a minimum later than nestin–/ChAT+ neurons in the medial septum-diagonal band of Broca. The results suggest that nestin+ cholinergic neurons may have a closer connection to olfactory bulb neurons. Nestin+ cholinergic neurons may have a stronger tolerance to injury than Nestin–/ChAT+ neurons. The difference between nestin+ and nestin–/ChAT+ neurons during the recovery process requires further investigations.

Key words: nerve regeneration, nestin, choline acetyl transferase, neurotransmitter, medial septum-diagonal band of Broca, olfactory bulb, olfactory nerve transection, the Guangdong Natural Science Foundation of China, neural regeneration