视神经损伤

    L- and T-type Ca2+ channels dichotomously contribute to retinal ganglion cell injury in experimental glaucoma
  • Figure 9|T-type Ca2+ channel blocker mibefradil reduces RGC apoptosis in COH retinas. 



    T-type VGCCs are low-voltage activated channels. Depolarization of cell membrane potential first activates these Ca2+ channels, resulting in Ca2+ influx (Qian et al., 2017). We previously showed that IOP elevation induced a depolarization of membrane potential in RGCs (Zhao et al., 2018), which activates T-type VGCCs, thus contributing to Ca2+ overload and RGC damage. We next examined the role of T-type VGCCs in apoptosis of RGCs in COH retinas. The T-type Ca2+ channel blocker mibefradil (2 μL, 3 μM) was intravitreally pre-injected in COH rats and the effect on RGC apoptosis was examined by TUNEL assays. As shown in Figure 9A and B, the number of TUNEL-positive RGCs was significantly increased at G2w in COH retinas (1359 ± 139, n = 8, P < 0.001) compared with controls (67 ± 11, n = 7), similar to our previous reports (Chen et al., 2011; Xu et al., 2020). Pre-injection of mibefradil significantly reduced the number of TUNEL-positive RGCs to 584 ± 42 (n = 8, P < 0.001 vs. G2w and P = 0.002 vs. controls) (Figure 9A and B). 


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  • 发布日期: 2023-01-12  浏览: 219
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