The calmodulin-dependent protein kinase II inhibitor KN-93 protects rat cerebral cortical neurons from N-methyl-D-aspartic acid-induced injury

doi:10.3969/j.issn.1673-5374.2013.02.002

Abstract

Abstract:

In this study, primary cultured cerebral cortical neurons of Sprague-Dawley neonatal rats were treated with 0.25, 0.5, and 1.0 μM calmodulin-dependent protein kinase II inhibitor KN-93 after 50 μM N-methyl-D-aspartic acid-induced injury. Results showed that, compared with N-methyl-D- aspartic acid-induced injury neurons, the activity of cells markedly increased, apoptosis was significantly reduced, leakage of lactate dehydrogenase decreased, and intracellular Ca²⁺ concentrations in neurons reduced after KN-93 treatment. The expression of caspase-3, phosphorylated calmodulin-dependent protein kinase II and total calmodulin-dependent protein kinase II protein decreased after KN-93 treatment. And the effect was apparent at a dose of 1.0 μM KN-93. Experimental findings suggest that KN-93 can induce a dose-dependent neuroprotective effect, and that the underlying mechanism may be related to the down-regulation of caspase-3 and calmodulin- dependent protein kinase II expression.

Key words: neural regeneration, brain injury, calmodulin-dependent protein kinase II, KN-93, N-methyl-D- aspartic acid, caspase-3, calcium ion, apoptosis, neuroprotection, grant-supported paper, photographs-containing paper, neuroregeneration

Xuewen Liu, Cui Ma, Ruixian Xing, Weiwei Zhang, Buxian Tian, Xidong Li, Qiushi Li, Yanhui Zhang. The calmodulin-dependent protein kinase II inhibitor KN-93 protects rat cerebral cortical neurons from N-methyl-D-aspartic acid-induced injury[J]. Neural Regeneration Research, 2013, 8(2): 111-120.

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