Hypoxia regulates reactive oxygen species levels in SHG-44 glioma cells

doi:10.3969/j.issn.1673-5374.2013.06.009

Abstract

Abstract:

In the present study, cultured human SHG-44 glioma cells were subjected to a hypoxic environment simulated using the CoCl2 method. Flow cytometry showed increased reactive oxygen species production in these cells. Real-time reverse transcription-PCR showed significantly increased hypoxia-inducible factor-1α mRNA expression in cells exposed to the hypoxic condition. The antioxidant N-acetylcysteine significantly inhibited reactive oxygen species production and reduced hypoxia-inducible factor-1α mRNA expression in normoxic and hypoxic groups, especially in the latter group. These findings indicate that hypoxia induces reactive oxygen species production and hypoxia-inducible factor-1α mRNA expression in human SHG-44 glioma cells, and that the antioxidant N-acetylcysteine can inhibit these changes.

Key words: neural regeneration, basic research, central nervous system, reactive oxygen species, hypoxia-inducible factor-1α, hypoxia, N-acetylcysteine, glioma, antioxidant, photographs-containing paper, neuroregeneraion

Haitao Jiang, Jiangtao Xie, Gaofeng Xu, Yongyong Su, Jinzheng Li, Mang Zhu. Hypoxia regulates reactive oxygen species levels in SHG-44 glioma cells[J]. Neural Regeneration Research, 2013, 8(6): 554-560.

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