Neural Regeneration Research ›› 2013, Vol. 8 ›› Issue (32): 3036-3046.doi: 10.3969/j.issn.1673-5374.2013.32.007

Previous Articles     Next Articles

A magnetic nanoparticles-based method for DNA extraction from the saliva of stroke patients

Li Yi1, Ying Huang1, Ting Wu2, Jun Wu3   

  1. Department of Neurology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong Province, China
  • Received:2013-06-24 Revised:2013-08-27 Online:2013-11-15 Published:2013-11-15
  • Contact: Li Yi, M.D., Associate professor, Chief physician, Department of Neurology, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong Province, China, yilitj@hotmail.com
  • About author:Li Yi and Ying Huang contributed equally to this article.
  • Supported by:

    This work was supported by the Scientific and Technological Innovation Foundation of Shenzhen, China, No. JC201105180757 and Shenzhen Science and Technology Projects, No. 201002063.

PDF

3142

Abstract:

C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene is a risk factor for stroke, suggesting that widespread detection could help to prevent stroke. DNA from 70 stroke pa-tients and 70 healthy controls was extracted from saliva using a magnetic nanoparticles-based method and from blood using conventional methods. Real-time PCR results revealed that the C677T polymorphism was genotyped by PCR using DNA extracted from both saliva and blood samples. The genotype results were confirmed by gene sequencing, and results for saliva and blood samples were consistent. The mutation TT genotype frequency was significantly higher in the stroke group than in controls. Homocysteine levels were significantly higher than controls in both TT genotype groups. Therefore, this noninvasive magnetic nanoparticles-based method using saliva samples could be used to screen for the MTHFR C677T polymorphism in target populations.

Key words: neural regeneration, brain injury, stroke, magnetic nanoparticles, saliva, methylenetetrahydrofolate reductase, homocysteine, gene polymorphism, gene screening, grants-supported paper, neuroregeneration