Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (5): 766-771.doi: 10.4103/1673-5374.157243

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Lateral intracerebroventricular injection of Apelin-13 inhibits apoptosis after cerebral ischemia/reperfusion injury

Xiao-ge Yan 1, Bao-hua Cheng 2, Xin Wang 2, Liang-cai Ding 2, Hai-qing Liu 3, Jing Chen 2, Bo Bai 2   

  1. 1 Xinxiang Medical University, Xinxiang, Henan Province, China
    2 Neurobiology Institute, Jining Medical University, Jining, Shandong Province, China
    3 Taishan Medical University, Taian, Shandong Province, China
  • Received:2015-02-12 Online:2015-05-15 Published:2015-05-15
  • Contact: Bo Bai, M.D., bbai@mail.jnmc.edu.cn
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of China, No. 30971081, 31271243, 81070961 and 81241052; and the Natural Science Foundation of Shandong Province of China, No. ZR2011CM027 and 2012GGA08100.

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Abstract:

Apelin-13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 (0.1 μg/g) was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immunohistochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immunoreactivity and decreased caspase-3 immunoreactivity. Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis.

Key words: nerve regeneration, brain injury, neuroprotection, cerebral ischemia/reperfusion injury, lateral intracerebroventricular injection, Apelin-13, nerve apoptosis, Bcl-2, caspase-3, NSFC grants, neural regeneration