Neural Regeneration Research ›› 2015, Vol. 10 ›› Issue (9): 1516-1522.doi: 10.4103/1673-5374.165526

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Optimal concentration and time window for proliferation and differentiation of neural stem cells from embryonic cerebral cortex: 5% oxygen preconditioning for 72 hours

Li-li Yuan1, 2, Ying-jun Guan2, Deng-dian Ma3, Hong-mei Du2   

  1. 1 Department of Histology and Embryology, Academy of Basic Medicine, Jining Medical University, Jining, Shandong Province, China
    2 Department of Histology and Embryology, Academy of Basic Medicine, Weifang Medical University, Weifang, Shandong Province, China
    3 Department of Otorhinolaryngology, Affiliated Hospital of Jining Medical University, Jining, Shandong Province, China
  • Received:2015-07-12 Online:2015-09-28 Published:2015-09-28
  • Contact: Li-li Yuan or Ying-jun Guan, Ph.D., liliyuan06@126.com or guanyj@wfmc.edu.cn.
  • Supported by:

    This work was supported by the Science Foundation of Jining Science and Technology Bureau of China, No. 2012jnjc07.

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Abstract:

Hypoxia promotes proliferation and differentiation of neural stem cells from embryonic day 12 rat brain tissue, but the concentration and time of hypoxic preconditioning are controversial. To address this, we cultured neural stem cells isolated from embryonic day 14 rat cerebral cortex in 5% and 10% oxygen in vitro. MTT assay, neurosphere number, and immunofluorescent staining found that 5% or 10% oxygen preconditioning for 72 hours improved neural stem cell viability and proliferation. With prolonged hypoxic duration (120 hours), the proportion of apoptotic cells increased. Thus, 5% oxygen preconditioning for 72 hours promotes neural stem cell proliferation and neuronal differentiation. Our findings indicate that the optimal concentration and duration of hypoxic preconditioning for promoting proliferation and differentiation of neural stem cells from the cerebral cortex are 5% oxygen for 72 hours.

Key words: nerve regeneration, brain injury, neural stem cells, low oxygen, cerebral cortex, apoptosis, differentiation, microtubule-associated protein 2, glial fibrillary acidic protein, caspase-3, neural regeneration