Neural Regeneration Research ›› 2016, Vol. 11 ›› Issue (10): 1638-1643.doi: 10.4103/1673-5374.193244

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Adipose-derived stem cells enhance myogenic di?erentiation in the mdx mouse model of muscular dystrophy via paracrine signaling

Ji-qing Cao1, 2, #, Ying-yin Liang1, 2, #, Ya-qin Li1, 2, Hui-li Zhang1, 2, Yu-ling Zhu3, Jia Geng4, Li-qing Yang1, Shan-wei Feng1, Juan Yang1, 2, Jie Kong1, 2, Cheng Zhang1, *   

  1. 1 Department of Neurology, First Afliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China 2 Guangdong Key Laboratory for the Diagnosis and Treatment of Major Neurological Disease, First Afliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China 3 First Afliated Hospital, Kunming Medical College, Kunming, Yunnan Province, China 4 Department of Neurology, Yantai Yuhuangding Hospital, Yantai, Shandong Province, China
  • Received:2016-09-01 Online:2016-10-31 Published:2016-10-31
  • Contact: Cheng Zhang, Ph.D., chengzhang100@hotmail.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 30370510, 30870851, 81271401; the Joint Fund of National Natural Science Foundation of China and Natural Science Foundation of Guangdong Province of China, No. U1032004.

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Abstract: Adipose-derived stem cells have been shown to promote peripheral nerve regeneration through the paracrine secretion of neurotrophic factors. However, it is unclear whether these cells can promote myogenic di?erentiation in muscular dystrophy. Adipose-derived stem cells (6 × 106) were injected into the gastrocnemius muscle of mdx mice at various sites. Dystrophin expression was found in the muscle fbers. Phosphorylation levels of Akt, mammalian target of rapamycin (mTOR), eIF-4E binding protein 1 and S6 kinase 1 were increased, and the Akt/mTOR pathway was activated. Simultaneously, myogenin levels were increased, whereas cleaved caspase 3 and vimentin levels were decreased. Necrosis and fbrosis were reduced in the muscle fbers. Tese fndings suggest that adipose-derived stem cells promote the regeneration and survival of muscle cells by inhibiting apoptosis and fbrosis, thereby alleviating muscle damage in muscular dystrophy.

Key words: nerve regeneration, Duchenne muscular dystrophy, adipose-derived stem cells, myogenic di?erentiation, paracrine pathway, dystrophin, neural regeneration