Neural Regeneration Research ›› 2023, Vol. 18 ›› Issue (5): 1147-1153.doi: 10.4103/1673-5374.353497

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Long noncoding RNA Pvt1 promotes the proliferation and migration of Schwann cells by sponging microRNA-214 and targeting c-Jun following peripheral nerve injury

Bin Pan1, #, Di Guo1, #, Li Jing2, Ke Li3, Xin Li1, Gen Li1, Xiao Gao1, Zhi-Wen Li4, Wei Zhao5, Hu Feng1, *, Meng-Han Cao6, *   

  1. 1Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China;  2Department of Orthopedics, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China; 3Department of Imaging, Xuzhou Central Hospital, Xuzhou, Jiangsu Province, China;  4College of Extended Education, Xuzhou Medical University, Xuzhou, Jiangsu Province, China;  5Department of Orthopedics, Kuitun Hospital, Yili Kazak Autonomous Prefecture, Xinjiang Uygur Autonomous Region, China; 6Center of Clinical Oncology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, China
  • Online:2023-05-15 Published:2022-11-01
  • Contact: Meng-Han Cao, MS, mhcao@xzhmu.edu.cn; Hu Feng, MS, xzfeng_hu@126.com.
  • Supported by:
    The study was supported by the National Natural Science Foundation of China, No. 81801213 (to BP); Xuzhou Special Fund for Promoting Scientific and Technological Innovation, Nos. KC21177 (to BP), KC21195 (to HF); and Science and Technology Project of Yili Kazak Autonomous Prefecture, No. YZ2019D006 (to HF). 

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Abstract: Research has shown that long-chain noncoding RNAs (lncRNAs) are involved in the regulation of a variety of biological processes, including peripheral nerve regeneration, in part by acting as competing endogenous RNAs. c-Jun plays a key role in the repair of peripheral nerve injury. However, the precise underlying mechanism of c-Jun remains unclear. In this study, we performed microarray and bioinformatics analysis of mouse crush-injured sciatic nerves and found that the lncRNA Pvt1 was overexpressed in Schwann cells after peripheral nerve injury. Mechanistic studies revealed that Pvt1 increased c-Jun expression through sponging miRNA-214. We overexpressed Pvt1 in Schwann cells cultured in vitro and found that the proliferation and migration of Schwann cells were enhanced, and overexpression of miRNA-214 counteracted the effects of Pvt1 overexpression on Schwann cell proliferation and migration. We conducted in vivo analyses and injected Schwann cells overexpressing Pvt1 into injured sciatic nerves of mice. Schwann cells overexpressing Pvt1 enhanced the regeneration of injured sciatic nerves following peripheral nerve injury and the locomotor function of mice was improved. Our findings reveal the role of lncRNAs in the repair of peripheral nerve injury and highlight lncRNA Pvt1 as a novel potential treatment target for peripheral nerve injury. 

Key words: cell migration, ceRNA, c-Jun, lncRNA, microarray, miR-214, nerve regeneration, peripheral nerve injury, Pvt1, Schwann cells