中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (9): 802-808.doi: 10.3969/j.issn.1673-5374.2013.09.004

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

电针降低内脏高敏感背根神经节中P2嘌呤受体的表达

  

  • 收稿日期:2012-06-18 修回日期:2013-02-22 出版日期:2013-03-25 发布日期:2013-03-25
  • 基金资助:

    国家自然科学基金项目（No.30973783）；上海市科委项目（No.11ZR1434300）；国家中医药管理局重点学科

Electroacupuncture diminishes P2X2 and P2X3 purinergic receptor expression in dorsal root ganglia of rats with visceral hypersensitivity

Zhijun Weng1, Luyi Wu1, Yuan Lu1, Lidong Wang1, Linying Tan1, Ming Dong1, Yuhu Xin2   

  1. 1 Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, China
    2 Fudan University Shanghai Cancer Center, Shanghai 200032, China
  • Received:2012-06-18 Revised:2013-02-22 Online:2013-03-25 Published:2013-03-25
  • Contact: Yuhu Xin, M.D., Technician-in- charge, Fudan University Shanghai Cancer Center, Shanghai 200032, China, yuhu_xin@ 163.com.
  • About author:Zhijun Weng★, Master. Zhijun Weng and Luyi Wu contributed equally to this paper.
  • Supported by:

    This project was funded by the National Natural Science Foundation of China, No. 30973783; the Shanghai Municipal Scientific Committee Project, No. 11ZR1434300; and the Key Subject Program of State Administration of Traditional Chinese Medicine.

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摘要：

研究已证实电针刺激上巨虚、天枢等穴能够改善大鼠内脏高敏感。实验采用结直肠球囊扩张法制备大鼠慢性内脏高敏感模型。应用免疫组化法检测肠易激综合征内脏高敏感大鼠背根神经节神经元中P2X2、P2X3受体表达。结果发现，内脏高敏感模型大鼠腹部撤回反射评分显著增高，即内脏敏感性升高，同时背根神经节神经元P2X2和P2X3受体异常增高；电针刺激双侧上巨虚和天枢后，内脏高敏感大鼠腹部撤回反射分和背根神经节神经元P2X2和P2X3受体表达降低。提示电针治疗可能通过降低背根神经节神经元中P2X2和P2X3受体的表达来改善肠易激综合征大鼠的内脏高敏感。

关键词: 神经再生, 针灸, P2X2, P2X3, 内脏高敏感, 肠易激综合征, 电针, P2嘌呤受体, 腹部撤回反射评分, 内脏敏感性, 中医, 外周神经损伤, 基金资助文章, 图片文章

Abstract:

Electroacupuncture at Shangjuxu (ST37) and Tianshu (ST25) can improve visceral hypersensitivity in rats. Colorectal distension was used to establish a rat model of chronic visceral hypersensitivity. Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. Results demonstrated that abdominal withdrawal reflex scores obviously increased following establishment of the model, indicating visceral hypersensitivity. Simultaneously, P2X2 and P2X3 receptor expression increased in dorsal root ganglia. After bilateral electroacupuncture at Shangjuxu and Tianshu, abdominal withdrawal reflex scores and P2X2 and P2X3 receptor expression decreased in rats with visceral hypersensitivity. These results indicated that electroacupuncture treatment improved visceral hypersensitivity in rats with irritable bowel syndrome by reducing P2X2 and P2X3 receptor expression in dorsal root ganglia.

Key words: neural regeneration, acupuncture and moxibustion, P2X2, P2X3, visceral hypersensitivity, irritable bowel syndrome, electroacupuncture, P2 purinergic receptors, abdominal withdrawal reflex scores, acupuncture and moxibustion, peripheral nerve injury, grants-supported paper, photographs- containing paper, neuroregeneration