中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (15): 1368-1375.doi: 10.3969/j.issn.1673-5374.2013.15.003

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

姜黄素减轻人类1型免疫缺陷病毒所致海马神经的损伤

  

  • 收稿日期:2012-10-15 修回日期:2013-03-29 出版日期:2013-05-25 发布日期:2013-05-25

Curcumin ameliorates hippocampal neuron damage induced by human immunodeficiency virus-1

Hongmei Tang1, 2, 3, Rui Pan4, Wenli Fang1, Yanyan Xing1, 2, 3, Dexi Chen5, Xiaobao Chen1, Yuanyuan Yu1, Junbing Wang1, 2, 3, Zheng Gong1, 2, 3, Guoyin Xiong1, 2, 3, Jun Dong1, 2, 3   

  1. 1 Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China
    2 Institute of Brain Research, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China
    3 Joint Laboratory for Brain Function and Health of Jinan University and the University of Hong Kong, Guangzhou 510632, Guangdong Province, China
    4 Department of Orthopedics, First Affiliated Hospital, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China
    5 STD/AIDS Research Lab, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China
  • Received:2012-10-15 Revised:2013-03-29 Online:2013-05-25 Published:2013-05-25
  • Contact: Jun Dong, Master, Doctoral supervisor, Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China; Institute of Brain Research, Medical College of Jinan University, Guangzhou 510632, Guangdong Province, China; Joint Laboratory for Brain Function and Health of Jinan University and the University of Hong Kong, Guangzhou 510632, Guangdong Province, China, donjunbox@163.com.
  • About author:Hongmei Tang★, Master. Hongmei Tang, Rui Pan and Wenli Fang contributed equally to this work.

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摘要：

作者前期的研究表明人类1型免疫缺陷病毒gp120V3 环可引起大鼠空间学习和记忆能力障碍及神经元损伤；姜黄素则可在一定程度上明显改善上述影响，而其具体机制尚不清楚。为此实验从海马脑片和海马神经元2个层面入手，进一步分析姜黄素的神经保护作用及机制。结果显示1 nmol/L gp120 V3环即可抑制海马长时程效应，并能抑制神经元突触的生长；而给予1μmol/L姜黄素后，上述情况得以改善。实验结果还显示姜黄素拮抗gp120致神经元损伤的作用是通过抑制神经元L-型钙电流的活化，从而减轻细胞内钙超载，同时可促进抗凋亡基因Bcl-2的表达，抑制凋亡基因Bax的活化而实现的，且效果与L-型钙通道拮抗剂尼莫地平相似，说明姜黄素确有可拮抗gp120 V3环诱导海马神经元损伤的效应。

关键词: 神经再生, 中医药, 姜黄素, 人类1型免疫缺陷病毒gp120 V3 环, 认知障碍, 海马脑片, 海马神经元, 钙离子, 长时程效应, 突触, 凋亡, 基金资助文章  
 

Abstract:

Our previous studies have shown that infection with the gp120 V3 loop can cause human immunodeficiency virus-1 associated neurocognitive disorders. Curcumin has been shown to improve these effects to some degree, but the precise mechanisms remain unknown. The present study analyzed the neuroprotective effect and mechanism of curcumin in relation to hippocampal neurons. Results showed that 1 nmol/L gp120 V3 loop suppressed the growth of synapses. After administration of 1 μmol/L curcumin, synaptic growth improved. Curcumin is neuroprotective against gp120 V3 loop-induced neuronal damage by inhibiting the activation of L-type calcium currents, relieving intracellular Ca2+ overload, promoting Bcl-2 expression, and inhibiting Bax activation. The effect of curcumin was identical to nimodipine, suggesting that curcumin has the same neuroprotective effects against gp120 V3 loop-induced neuronal damage.