中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (17): 1551-1559.doi: 10.3969/j.issn.1673-5374.2013.17.002
慢病毒携带Atoh1基因感染正常大鼠耳蜗
Lentivirus carrying the Atoh1 gene infects normal rat cochlea
Song Pan1, Jingzhi Wan2, Shaosheng Liu3, Song Zhang1, Hao Xiong4, Jun Zhou1, Wu Xiong1, Kunfei Yu1, Yong Fu5
- 1 Department of Otolaryngology, Guangming New District People’s Hospital, Shenzhen 518106, Guangdong Province, China
2 Department of Surgery, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China
3 Department of Otolaryngology, Beilun Branch of the First Affiliated Hospital, College of Medicine, Zhejiang University, Ningbo 315800, Zhejiang Province, China
4 Department of Otolaryngology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
5 Department of Otolaryngology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang Province, China
摘要：
携带Atoh1的腺病毒可以感染耳蜗柯替氏器，并在支持细胞区出现类似毛细胞的表面标准物，但腺病毒携带的基因表达属于瞬时表达，这无疑限制了其临床应用，而慢病毒是一种可以稳定、持续表达目的基因的载体。实验以携带Atoh1的慢病毒通过圆窗途径导入大鼠耳蜗30d后，大鼠耳蜗内的结构及听功能无明显影响，且携带的Atoh1基因使耳蜗支持细胞区生成毛细胞样细胞，并表达毛细胞表面标志物Myosin7a。说明慢病毒对耳蜗毛细胞和听功能没有明显危害，慢病毒携带的目的基因可持久感染正常大鼠耳蜗，作用安全有效。