中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (22): 2030-2038.doi: 10.3969/j.issn.1673-5374.2013.22.002

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

吴茱萸次碱对脑缺血再灌注的神经保护

  

  • 收稿日期:2013-03-19 修回日期:2013-06-27 出版日期:2013-08-05 发布日期:2013-08-05

Neuroprotective effects of rutaecarpine on cerebral ischemia reperfusion injury

Chunlin Yan, Ji Zhang, Shu Wang, Guiping Xue, Yong Hou   

  1. Department of Pharmacology, Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Received:2013-03-19 Revised:2013-06-27 Online:2013-08-05 Published:2013-08-05
  • Contact: Chunlin Yan, Master, Lecturer, Department of Pharmacology, Hebei North University, Zhangjiakou 075000, Hebei Province, China, yanchunlinycl@ 163.com.
  • Supported by:

    张家口市科技项目指导计划项目(1021095D)；2010年河北北方学院校级课题资助No:Q2010018

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摘要：

已有研究表明中药吴茱萸的活性成分吴茱萸次碱可改善心肌缺血再灌注损伤。鉴于缺血性心脑血管疾病同属血管病变，关系极为密切，实验设想茱萸次碱对脑缺血再灌注损伤也有神经保护作用。给鼠每日腹腔注射84，252，504 µg/kg 的吴茱萸次碱连续7 d，建立脑缺血再灌注小鼠模型。避暗实验、斜板实验、TTC染色、氧化应激相关指标检测结果显示，吴茱萸次碱可以减轻脑缺血再灌注损伤小鼠的学习记忆障碍和神经功能症状，减少脑梗死面积和脑含水量，降低部分脑组织丙二醛含量，提高脑组织超氧化物歧化酶及谷胱甘肽过氧化物酶活性。由此认为，吴茱萸次碱可以明显改善脑缺血再灌注损伤造成的神经功能损伤，其机制可能与氧化应激有关。结果验证了课题组的假设。

关键词: 神经再生, 中医药, 吴茱萸次碱, 脑缺血再灌注, 学习记忆, 神经功能损伤, 脑梗死面积, 自由基, 谷胱甘肽过氧化物酶, 超氧化物歧化酶, 丙二醛, 基金资助文章

Abstract:

Rutaecarpine, an active component of the traditional Chinese medicine Tetradium ruticarpum, has been shown to improve myocardial ischemia reperfusion injury. Because both cardiovascular and cerebrovascular diseases are forms of ischemic vascular disease, they are closely related. We hypothesized that rutaecarpine also has neuroprotective effects on cerebral ischemia reperfusion injury. A cerebral ischemia reperfusion model was established after 84, 252 and 504 μg/kg rutaecarpine were given to mice via intraperitoneal injection, daily for 7 days. Results of the step through test, 2,3,5-triphenyl tetrazolium chloride dyeing and oxidative stress indicators showed that rutaecarpine could improve learning and memory ability, neurological symptoms and reduce infarc-tion volume and cerebral water content in mice with cerebral ischemia reperfusion injury. Rutaecarpine could significantly decrease the malondialdehyde content and increase the activities of superoxide dismutase and glutathione peroxidase in mouse brain. Therefore, rutaecarpine could improve neurological function following injury induced by cerebral ischemia reperfusion, and the mechanism of this improvement may be associated with oxidative stress. These results verify that rutaecarpine has neuroprotective effects on cerebral ischemia reperfusion in mice.

Key words: neural regeneration, traditional Chinese medicine, rutaecarpine, cerebral ischemia reperfusion, learning and memory, infarct volume, free radical, glutathione peroxidase, superoxide dismutase, malondialdehyde, grants-supported paper, neuroregeneration