中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (32): 3003-3012.doi: 10.3969/j.issn.1673-5374.2013.32.003

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

加巴喷丁抑制偏头痛中枢敏感化的机制

  

  • 收稿日期:2013-06-07 修回日期:2013-09-15 出版日期:2013-11-15 发布日期:2013-11-15
  • 基金资助:

    山东省自然科学基金(No.ZR2012HQ014)；山东省高等学校科技计划(No. J10LF14)

Gabapentin inhibits central sensitization during migraine

Yanbo Zhang1, 2, Guo Shao3, Wei Zhang4, Sijie Li1, Jingzhong Niu2, Dongmei Hu2, Mingfeng Yang2, Xunming Ji1   

  1. 1 Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
    2 Department of Neurology, Affiliated Hospital of Taishan Medical University, Tai’an 271000, Shandong Province, China
    3 Biomedical Research Center, Baotou Medical College, Baotou 014060, Inner Mongolia Autonomous Region, China
    4 Department of Gastroenterology, Second Affiliated Hospital of Baotou Medical College, Baotou 014010, Inner Mongolia Autonomous Region, China
  • Received:2013-06-07 Revised:2013-09-15 Online:2013-11-15 Published:2013-11-15
  • Contact: Xunming Ji, Ph.D., Doctoral supervisor, Professor, Chief physician, Institute of Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100053, China, jixm70@hotmail.com.
  • About author:Yanbo Zhang, Master, Associate professor.
  • Supported by:

    This study was financially sponsored by the Natural Science Foundation of Shandong Province, No. ZR2012HQ014, and a grant from the Science and Technology Plan Program of Universities in Shandong Province, No. J10LF14.

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摘要：

Peripheral and central sensitizations are phenomena that occur during migraine. The role of gabapentin, a migraine preventive drug, on central sensitization remains unclear. In this study, a rat model of migraine was established by electrical stimulation of the trigeminal ganglion, and the an-imals were given intragastric gabapentin. Changes in amino acid content in the cerebrospinal fluid and protein kinase C membrane translocation in the spinal trigeminal nucleus were examined to clarify the mechanisms underlying the efficacy of gabapentin in the treatment of central sensitization during migraine. Electrophysiology, liquid chromatography-mass spectrometry and western blot analysis results revealed that gabapentin reduces neuronal excitability in the spinal nucleus in the trigeminal nerve, decreases excitatory amino acid content and inhibits the activation of protein ki-nase C. This provides evidence that excitatory amino acids and protein kinase C are involved in the formation and maintenance of central sensitization during migraine. Gabapentin inhibits migraine by reducing excitatory amino acid content in the cerebrospinal fluid and inhibiting protein kinase C ac-tivation.

关键词: neural regeneration, migraine, protein kinase C, excitatory amino acid, ionic excitatory amino acid receptors, neurons, calcitonin gene-related peptide, nitric oxide synthase, nuclei of trigeminal nerve, glutamic acid, gamma-aminobutyric acid, grants-supported paper, neuroregeneration

Abstract:

Peripheral and central sensitizations are phenomena that occur during migraine. The role of gabapentin, a migraine preventive drug, on central sensitization remains unclear. In this study, a rat model of migraine was established by electrical stimulation of the trigeminal ganglion, and the an-imals were given intragastric gabapentin. Changes in amino acid content in the cerebrospinal fluid and protein kinase C membrane translocation in the spinal trigeminal nucleus were examined to clarify the mechanisms underlying the efficacy of gabapentin in the treatment of central sensitization during migraine. Electrophysiology, liquid chromatography-mass spectrometry and western blot analysis results revealed that gabapentin reduces neuronal excitability in the spinal nucleus in the trigeminal nerve, decreases excitatory amino acid content and inhibits the activation of protein ki-nase C. This provides evidence that excitatory amino acids and protein kinase C are involved in the formation and maintenance of central sensitization during migraine. Gabapentin inhibits migraine by reducing excitatory amino acid content in the cerebrospinal fluid and inhibiting protein kinase C ac-tivation.

Key words: neural regeneration, migraine, protein kinase C, excitatory amino acid, ionic excitatory amino acid receptors, neurons, calcitonin gene-related peptide, nitric oxide synthase, nuclei of trigeminal nerve, glutamic acid, gamma-aminobutyric acid, grants-supported paper, neuroregeneration