中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (32): 2991-3002.doi: 10.3969/j.issn.1673-5374.2013.32.002

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

褪黑素受体1在三叉神经尾侧脊束核的分布及效应

  

  • 收稿日期:2012-06-20 修回日期:2013-09-11 出版日期:2013-11-15 发布日期:2013-11-15
  • 基金资助:

    国家自然科学基金项目(No.81271166, No.81371107)；广东省自然科学基金项目(No.10451008901006145)

Orofacial inflammatory pain affects the expression of MT1 and NADPH-d in rat caudal spinal trigeminal nucleus and trigeminal ganglion

Fang Huang, Hongwen He, Wenguo Fan, Yongliang Liu, Hongyu Zhou, Bin Cheng   

  1. Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, Guangdong Province, China
  • Received:2012-06-20 Revised:2013-09-11 Online:2013-11-15 Published:2013-11-15
  • Contact: Bin Cheng, Ph.D., Professor, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, Guangdong Province, China, chengbin@mail.sysu.edu.cn.
  • About author:Fang Huang, Ph.D., Associate chief physician.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81271166, 81371107; the Natural Science Foundation of Guangdong Province in China, No. 10451008901006145.

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摘要：

在三叉感觉通路中有关褪黑素作用的研究较少，其中褪黑素受体1的研究更少。实验在成年大鼠右上唇触须垫处注射甲醛，建立颌面炎性疼痛模型后，应用免疫组化方法首次定位了褪黑素受体1在三叉神经尾侧脊束核的分布，发现了颌面炎性痛刺激早期在三叉神经节和三叉神经尾侧脊束核神经元内褪黑素受体1和还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶表达存在明显的组织差异性，验证了当褪黑素受体1在三叉神经尾侧脊束核的表达明显下调时，褪黑素在三叉神经尾侧脊束核水平经褪黑素受体1调控痛觉作用减弱的特点。

关键词: 神经再生, 疼痛, 褪黑素, 一氧化氮, 颌面疼痛, 三叉神经尾侧脊束核, 三叉神经节, 三叉神经中脑核, 褪黑素受体1, 还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶, 基金资助文章

Abstract:

Very little is known about the role of melatonin in the trigeminal system, including the function of melatonin receptor 1. In the present study, adult rats were injected with formaldehyde into the right vibrissae pad to establish a model of orofacial inflammatory pain. The distribution of melatonin re-ceptor 1 and nicotinamide adenine dinucleotide phosphate diaphorase in the caudal spinal trigem-inal nucleus and trigeminal ganglion was determined with immunohistochemistry and histochemistry. The results show that there are significant differences in melatonin receptor 1 expression and nicotinamide adenine dinucleotide phosphate diaphorase expression in the trigeminal ganglia and caudal spinal nucleus during the early stage of orofacial inflammatory pain. Our findings suggest that when melatonin receptor 1 expression in the caudal spinal nucleus is significantly reduced, melatonin’s regulatory effect on pain is attenuated.

Key words: neural regeneration, pain, melatonin, nitric oxide, maxillofacial pain, caudal spinal trigeminal nucleus, trigeminal ganglia, mesencephalic trigeminal nucleus, melatonin receptor 1, nicotinamide adenine dinucleotide phosphate diaphorase, grants-supported paper, neuroregeneration