中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (1): 33-40.doi: 10.4103/1673-5374.125327

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

含胶质细胞源性神经营养因子载体可促进向神经元样细胞分化

  

  • 收稿日期:2013-11-30 出版日期:2014-01-05 发布日期:2014-01-05

Transfection of the glial cell line-derived neurotrophic factor gene promotes neuronal differentiation

Jie Du1, 2, Xiaoqing Gao3, Li Deng3, Nengbin Chang2, Huailin Xiong2, Yu Zheng1   

  • Received:2013-11-30 Online:2014-01-05 Published:2014-01-05
  • Contact: Yu Zheng, Ph.D., Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan Province, China, yzheng@scu.edu.cn

摘要:

用含有维甲酸和表皮细胞生长因子的培养基诱导胶质细胞源性神经营养因子重组腺病毒载体感染大鼠骨髓间充质干细胞后,观察细胞活性、微管相关蛋白2阳性细胞率、细胞上清液中胶质细胞源性神经营养因子,神经生长因子和生长相关蛋白43和细胞中微管相关蛋白2、胶质细胞源性神经营养因子,神经生长因子和生长相关蛋白43mRNA的表达水平,明显高于以空病毒质粒感染的大鼠骨髓间充质干细胞。说明胶质细胞源性神经营养因子重组腺病毒载体感染的大鼠骨髓间充质干细胞较空病毒质粒感染的大鼠骨髓间充质干细胞有更高的神经元样细胞分化比例,其分化机制可能与通过上调胶质细胞源性神经营养因子、神经生长因子和生长相关蛋白43的表达有关。

关键词: 神经再生, 骨髓间充质干细胞, 细胞分化, 神经元样, 胶质细胞源性神经营养因子, 重组腺病毒载体, 感染的, 维甲酸, 表皮细胞生长因子, 神经生长因子, 生长相关蛋白43

Abstract:

Glial cell line-derived neurotrophic factor recombinant adenovirus vector-transfected bone marrow mesenchymal stem cells were induced to differentiate into neuron-like cells using inductive medium containing retinoic acid and epidermal growth factor. Cell viability, microtubule-associated protein 2-positive cell ratio, and the expression levels of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 protein in the supernatant were significantly higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. Furthermore, microtubule-associated protein 2, glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43 mRNA levels in cell pellets were statistically higher in glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells compared with empty virus plasmid-transfected bone marrow mesenchymal stem cells. These results suggest that glial cell line-derived neurotrophic factor/bone marrow mesenchymal stem cells have a higher rate of induction into neuron-like cells, and this enhanced differentiation into neuron-like cells may be associated with up-regulated expression of glial cell line-derived neurotrophic factor, nerve growth factor and growth-associated protein-43.

Key words: nerve regeneration, bone marrow mesenchymal stem cells, cell differentiation, neuron-like cells, glial cell line-derived neurotrophic factor, recombinant adenovirus vector, transfection, retinoic acid, epidermal growth factor, nerve growth factor, growth-associated protein-43, neural regeneration