中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (10): 1075-1078.doi: 10.4103/1673-5374.133168

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

去乙酰甲壳素导管桥接与电刺激并用修复损伤的周围神经

  

  • 收稿日期:2014-04-20 出版日期:2014-05-26 发布日期:2014-05-26
  • 基金资助:

    国家重点基础研究发展计划（973 计划）（2014CB542200），国家自然科学基金（31171150，31271284，30801169），教育部新世纪优秀人才支持计划（BMU20110270），北京市科技新星计划（2008A010），教育院高等院校博士点新教师基金（20070001780）

Bridging peripheral nerves using a deacetyl chitin conduit combined with short-term electrical stimulation

Zhongli Zhang 1, Xin Li 2, Songjie Zuo 3, Jie Xin 1, Peixun Zhang 4   

  1. 1 Department of Traumatic Orthopedics, Weifang People’s Hospital, Weifang, Shandong Province, China
    2 Department of Physiology, Weifang Medical College, Weifang, Shandong Province, China
    3 National Institute for Radiological Protection, Chinese Center for Disease Prevention and Control, Beijing, China
    4 Department of Trauma & Orthopedics, Peking University People’s Hospital, Beijing, China
  • Received:2014-04-20 Online:2014-05-26 Published:2014-05-26
  • Contact: Peixun Zhang, M.D., Ph.D., Department of Trauma & Orthopedics, Peking University People’s Hospital, Beijing 100044, China,zhangpeixun@bjmu.edu.cn.
  • Supported by:

    This study was funded by National Program on Key Basic Research Project of China (973 Program), No. 2014CB542200; the National Natural Science Foundation of China, No. 31171150, 31271284, 30801169; the Chinese Educational Ministry New Century Excellent Talent Support Project, No. BMU20110270; the Beijing City Science & Technology New Star Classification, No. 2008A010; and the Ministry of Education New Teachers of Institutions of Higher Learning Doctoral Fund, No. 20070001780.

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摘要：

既往研究显示单纯使用去乙酰甲壳素导管桥接或电刺激均能有效促进损伤周围神经的再生。我们假设两者复合对周围神经的损伤修复效果更佳，实验发现对右侧坐骨神经横断损伤大鼠同时使用乙酰甲壳素导管桥接和电刺激(0.1ms, 3V, 20Hz, 持续1h)干预，在其后的6及12周，大鼠坐骨神经运动神经传导速度、有髓神经纤维数量、纤维直径、轴突直径和髓鞘厚度均明显高（多）于单纯使用乙酰甲壳素导管桥接未进行电刺激的大鼠。结果显示去乙酰甲壳素导管桥接与短暂电刺激并用修复周围神经损伤的效果更好。

Abstract:

Previous studies have demonstrated that deacetyl chitin conduit nerve bridging or electrical stimulation can effectively promote the regeneration of the injured peripheral nerve. We hypothesized that the combination of these two approaches could result in enhanced regeneration. Rats with right sciatic nerve injury were subjected to deacetyl chitin conduit bridging combined with electrical stimulation (0.1 ms, 3 V, 20 Hz, for 1 hour). At 6 and 12 weeks after treatment, nerve conduction velocity, myelinated axon number, fiber diameter, axon diameter and the thickness of the myelin sheath in the stimulation group were better than in the non-stimulation group. The results indicate that deacetyl chitin conduit bridging combined with temporary electrical stimulation can promote peripheral nerve repair.

Key words: nerve regeneration, peripheral nerve injury, deacetyl chitin conduit, electrical stimulation, NSFC grant, neural regeneration