关键词: 神经再生, 碘125, 镇痛, 放射性粒子, 超微结构, 疼痛阈值, 背根神经节损伤, 神经病理性疼痛

Abstract:

The use of iodine-125 (125I) in cancer treatment has been shown to relieve patients’ pain. Considering dorsal root ganglia are critical for neural transmission between the peripheral and central nervous systems, we assumed that 125I could be implanted into rat dorsal root ganglia to provide relief for neuropathic pain. 125I seeds with different radioactivity (0, 14.8, 29.6 MBq) were implanted separately through L4–5 and L5–6 intervertebral foramen into the vicinity of the L5 dorsal root ganglion. von Frey hair results demonstrated the mechanical pain threshold was elevated after implanting 125I seeds from the high radioactivity group. Transmission electron microscopy revealed that nuclear membrane shrinkage, nucleolar margination, widespread mitochondrial swelling, partial vacuolization, lysosome increase, and partial endoplasmic reticulum dilation were visible at 1,440 hours in the low radioactivity group and at 336 hours in the high radioactivity group. Abundant nuclear membrane shrinkage, partial fuzzy nuclear membrane and endoplasmic reticulum necrosis were observed at 1,440 hours in the high radioactivity group. No significant difference in combined behavioral scores was detected between preoperation and postoperation in the low and high radioactivity groups. These results suggested that the mechanical pain threshold was elevated after implanting 125I seeds without influencing motor functions of the hind limb, although cell injury was present.

Key words: nerve regeneration, iodine-125, analgesia, radioactive seeds, ultrastructure, pain threshold, dorsal root ganglion injury, neuropathic pain, neural regeneration