中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (22): 1961-1967.doi: 10.4103/1673-5374.145374

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

背根神经节来源许旺细胞复合聚乳酸-壳聚糖神经导管修复坐骨神经缺损

  

  • 收稿日期:2014-06-03 出版日期:2014-11-25 发布日期:2014-11-25

Dorsal root ganglion-derived Schwann cells combined with poly(lactic-co-glycolic acid)/chitosan conduits for the repair of sciatic nerve defects in rats

Li Zhao 1, Wei Qu 2, Yuxuan Wu 2, Hao Ma 2, Huajun Jiang 2   

  1. 1 Department of Anesthesiology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, Shanxi Province, China
    2 Department of Orthopedics, First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning Province, China
  • Received:2014-06-03 Online:2014-11-25 Published:2014-11-25
  • Contact: Huajun Jiang, Ph.D., Department of Orthopedics, First Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China, jianghuajun112@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30973060.

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摘要：

目前分离许旺细胞容易受到成纤维细胞的污染，且成本高效率低。实验收集新生小鼠的脊髓背根神经节，酶消化分散后以无血清的黑素细胞培养基纯化许旺细胞，其获得的许旺细胞纯度可达95%以上，远高于含胎牛血清的DMEM培养基提取的许旺细胞。这些许旺细胞复合聚乳酸-羟基乙酸共聚物/壳聚糖导管修复大鼠坐骨神经10mm缺损，发现大鼠坐骨神经组织轴突直径及轴突面积显著增加，运动功能明显改善，表明无血清的黑素细胞培养基更容易纯化许旺细胞，且许旺细胞复合聚乳酸-羟基乙酸共聚物/壳聚糖神经导管可有效用于修复坐骨神经缺损。

关键词: 神经再生, 许旺细胞, 背根神经节, 黑素细胞培养基, 成纤维细胞, 聚乳酸-羟基乙酸共聚物, 壳聚糖, 坐骨神经缺损, NSFC grant

Abstract:

Schwann cells, nerve regeneration promoters in peripheral nerve tissue engineering, can be used to repair both the peripheral and central nervous systems. However, isolation and purification of Schwann cells are complicated by contamination with fibroblasts. Current reported measures are mainly limited by either high cost or complicated procedures with low cell yields or purity. In this study, we collected dorsal root ganglia from neonatal rats from which we obtained highly purified Schwann cells using serum-free melanocyte culture medium. The purity of Schwann cells (> 95%) using our method was higher than that using standard medium containing fetal bovine serum. The obtained Schwann cells were implanted into poly(lactic-co-glycolic acid)/chitosan conduits to repair 10-mm sciatic nerve defects in rats. Results showed that axonal diameter and area were significantly increased and motor functions were obviously improved in the rat sciatic nerve tissue. Experimental findings suggest that serum-free melanocyte culture medium is conducive to purify Schwann cells and poly(lactic-co-glycolic acid)/chitosan nerve conduits combined with Schwann cells contribute to restore sciatic nerve defects.

Key words: nerve regeneration, Schwann cells, dorsal root ganglia, melanocyte medium, fibroblasts, poly(lactic-co-glycolic acid), chitosan, sciatic nerve defect, NSFC grants, neural regeneration