中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (4): 594-598.doi: 10.4103/1673-5374.155433

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

创伤性脑损伤昏迷大鼠促醒:正中神经电刺激的修复机制

  

  • 收稿日期:2014-11-22 出版日期:2015-04-22 发布日期:2015-04-22
  • 基金资助:

    中国国家自然科学基金项目(81260295);江西省自然科学基金项目(20132BAB205063)

Resuscitation therapy for traumatic brain injury-induced coma in rats: mechanisms of median nerve electrical stimulation

Zhen Feng, Ying-jun Zhong, Liang Wang, Tian-qi Wei   

  1. Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China
  • Received:2014-11-22 Online:2015-04-22 Published:2015-04-22
  • Contact: Zhen Feng, M.D., fengzhenly@sina.com
  • Supported by:

    This study was funded by grants from the National Natural Science Foundation of China, No. 81260295, and the Natural Science Foundation of Jiangxi Province of China, No. 20132BAB205063.

摘要:

实验设计了采用正中神经电刺激方法对创伤性脑损伤后昏迷状态的大鼠模型进行促醒,发现其促醒效果明显。刺激后大鼠前额叶皮质中orexin-A及其受体OX1R表达增加,且orexin-A的表达随时间延长而逐渐增加,而OX1R在12h达到峰值后减少。电刺激前脑室注射OX1R拮抗剂SB334867的创伤性脑损伤大鼠,苏醒大鼠数量减少,且前额叶皮质中orexin-A及OX1R表达减少。说明正中神经电刺激诱导昏迷大鼠前额叶皮质中orexin-A及OX1R表达参与了促醒的过程中。

关键词: 神经再生, 创伤性脑损伤, 昏迷, 正中神经电刺激, 促醒, Orexin-A, OX1R

Abstract:

In this study, rats were put into traumatic brain injury-induced coma and treated with median nerve electrical stimulation. We explored the wake-promoting effect, and possible mechanisms, of median nerve electrical stimulation. Electrical stimulation upregulated the expression levels of orexin-A and its receptor OX1R in the rat prefrontal cortex. Orexin-A expression gradually increased with increasing stimulation, while OX1R expression reached a peak at 12 hours and then decreased. In addition, after the OX1R antagonist, SB334867, was injected into the brain of rats after traumatic brain injury, fewer rats were restored to consciousness, and orexin-A and OXIR expression in the prefrontal cortex was downregulated. Our findings indicate that median nerve electrical stimulation induced an up-regulation of orexin-A and OX1R expression in the prefrontal cortex of traumatic brain injury-induced coma rats, which may be a potential mechanism involved in the wake-promoting effects of median nerve electrical stimulation.

Key words: nerve regeneration, traumatic brain injury, coma, median nerve electrical stimulation, wake-promoting, orexin-A, OX1R, NSFC grants, neural regeneration