中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (5): 753-759.doi: 10.4103/1673-5374.156971

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

经人参皂苷Rg1诱导分化的神经干细胞移植对缺氧缺血性脑病的神经保护效应

  

  • 收稿日期:2015-01-10 出版日期:2015-05-15 发布日期:2015-05-15

Neuroprotective effects of ginsenoside Rg1-induced neural stem cell transplantation on hypoxic-ischemic encephalopathy

Ying-bo Li, Yan Wang, Ji-ping Tang, Di Chen, Sha-li Wang   

  1. 1 Institute of Neuroscience, Chongqing Medical University, Chongqing, China
    2 Department of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA, USA
  • Received:2015-01-10 Online:2015-05-15 Published:2015-05-15
  • Contact: Sha-li Wang, M.D., 63557408@qq.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Chongqing in China, No. CSTC2011jjA0013.

摘要:

为了解人参皂苷Rg1是否能诱导神经干细胞分化及诱导后的神经干细胞移植是否对缺氧缺血性脑病有治疗效果。实验首先以10-80 μmol/mL的Rg1诱导神经干细胞分化,通过免疫组化染色发现20μmol/mL的Rg1具有最有效的诱导分化作用,且全细胞膜片钳结果也证实该剂量Rg1诱导分化的神经干细胞比未诱导细胞更为成熟,然后以该剂量Rg1诱导分化的神经干细胞侧脑室注射移植,治疗线栓法建立的缺氧缺血性脑病新生模型大鼠。结果证实,模型大鼠脑组织病理损伤明显减轻,行为能力明显改善,且发现移植的神经干细胞表达NSE,且主要分布于大脑海马及皮质组织中。表明移植Rg1诱导的神经干细胞发挥了促进损伤缺氧缺血性脑病模型大鼠功能恢复的神经保护效应。

关键词: 神经再生, 缺氧缺血性脑损伤, 人参皂苷Rg1, 神经干细胞, 细胞移植, 细胞分化, 认知, 神经重建

Abstract:

Ginsenoside Rg1 is the major pharmacologically active component of ginseng, and is reported to have various therapeutic actions. To determine whether it induces the differentiation of neural stem cells, and whether neural stem cell transplantation after induction has therapeutic effects on hypoxic-ischemic encephalopathy, we cultured neural stem cells in 10–80 μM ginsenoside Rg1. Immunohistochemistry revealed that of the concentrations tested, 20 mM ginsenoside Rg1 had the greatest differentiation-inducing effect and was the concentration used for subsequent experiments. Whole-cell patch clamp showed that neural stem cells induced by 20 μM ginsenoside Rg1 were more mature than non-induced cells. We then established neonatal rat models of hypoxic-ischemic encephalopathy using the suture method, and ginsenoside Rg1-induced neural stem cells were transplanted via intracerebroventricular injection. These tests confirmed that neural stem cells induced by ginsenoside had fewer pathological lesions and had a significantly better behavioral capacity than model rats that received saline. Transplanted neural stem cells expressed neuron-specific enolase, and were mainly distributed in the hippocampus and cerebral cortex. The present data suggest that ginsenoside Rg1-induced neural stem cells can promote the partial recovery of complicated brain functions in models of hypoxic-ischemic encephalopathy.

Key words: nerve regeneration, hypoxic-ischemic brain damage, ginsenoside Rg1, neural stem cells, cell transplantation, cell differentiation, cognition, nerve reconstruction, neural regeneration