中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (5): 772-777.doi: 10.4103/1673-5374.156979

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

CX3趋化因子受体1缺失成体小鼠海马新生神经元树突复杂性降低及成熟延迟

  

  • 收稿日期:2015-02-16 出版日期:2015-05-15 发布日期:2015-05-15

CX3 chemokine receptor 1 deficiency leads to reduced dendritic complexity and delayed maturation of newborn neurons in the adult mouse hippocampus

Feng Xiao, Jun-mei Xu, Xing-hua Jiang   

  1. Department of Anesthesiology, the Second Xiangya Hospital of Central South University, Changsha, Hunan Province, China
  • Received:2015-02-16 Online:2015-05-15 Published:2015-05-15
  • Contact: Xing-hua Jiang, M.D., Jiangxh1997@aliyun.com.

摘要:

既往研究显示小胶质细胞通过fractalkine/CX3趋化因子受体1信号通路积极参与海马神经再生的增殖和分化过程。但是小胶质细胞是否可以影响海马神经再生中新生神经元的成熟及树突生长目前还不清楚,实验希望揭示着其中的一些问题,我们发现CX3趋化因子受体1缺失(CX3CR1 GFP/GFP)转基因小鼠海马小胶质细胞中doublecortin阳性细胞数量减少,海马新生神经元树突的长度缩短,交叉点数量减少;使用海人酸诱发CX3CR1 GFP/GFP小鼠癫痫,发现神经元中完全成熟及完整性标志物即早期基因c-fos表达较少,说明CX3趋化因子受体1还可延迟海马新生神经元功能的成熟。

关键词: 神经再生, Fractalkine, CX3趋化因子受体1, 神经元成熟, 树突, Doublecortin, 突触成熟, 新生神经元

Abstract:

Previous studies have shown that microglia impact the proliferation and differentiation of neurons during hippocampal neurogenesis via the fractalkine/CX3 chemokine receptor 1 (CX3CR1) signaling pathway. However, whether microglia can influence the maturation and dendritic growth of newborn neurons during hippocampal neurogenesis remains unclear. In the present study, we found that the number of doublecortin-positive cells in the hippocampus was decreased, and the dendritic length and number of intersections in newborn neurons in the hippocampus were reduced in transgenic adult mice with CX3CR1 deficiency (CX3CR1GFP/GFP). Furthermore, after experimental seizures were induced with kainic acid in these CX3CR1-deficient mice, the expression of c-fos, a marker of neuronal activity, was reduced compared with wild-type mice. Collectively, the experimental findings indicate that the functional maturation of newborn neurons during hippocampal neurogenesis in adult mice is delayed by CX3CR1 deficiency.

Key words: nerve regeneration, fractalkine, CX3 chemokine receptor 1, neuronal maturation, dendrites, doublecortin, synaptic maturation, newborn neurons, neural regeneration