中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (6): 989-995.doi: 10.4103/1673-5374.158366

• 原著:颅神经损伤修复保护与再生 • 上一篇    下一篇

鼓索神经切断后同侧味蕾萎缩而对侧味蕾增生

  

  • 收稿日期:2015-05-16 出版日期:2015-06-18 发布日期:2015-06-18

Shrinkage of ipsilateral taste buds and hyperplasia of contralateral taste buds following chorda tympani nerve transection

Yi-ke Li 1, 2, 3, 4, Juan-mei Yang 1, 2, 3, Yi-bo Huang 1, 2, 3, Dong-dong Ren 1, 2, 3,Fang-lu Chi 1, 2, 3   

  1. 1 Department of Otology and Skull Base Surgery, Eye Ear Nose & Throat Hospital, Fudan University, Shanghai, China
    2 Shanghai Auditory Medical Center, Shanghai, China
    3 Key Laboratory of Hearing Science, Ministry of Health, Shanghai, China
    4 Department of Hearing and Speech Sciences, Vanderbilt University, Nashville, TN, USA
  • Received:2015-05-16 Online:2015-06-18 Published:2015-06-18
  • Contact: Fang-lu Chi, M.D., Ph.D. or Dong-dong Ren, M.D., Ph.D., chifanglu@126.com or dongdongren@fudan.edu.cn.
  • Supported by:

     This research was supported by the Major State Basic Research Development Program of China (973 Program), No. 2011CB504500, 2011CB504506; the National Natural Science Foundation of China, No. 81271084, 81420108010, 81000413, 81370022, 81200740; the Training Program of the Excellent Young Talents of the Shanghai Municipal Health System in China, No. XYQ2013084; the Innovation Project of Shanghai Municipal Science and Technology Commission in China, No. 11411952300.

摘要:

目前还没有对失神经支配后味蕾形态随时间变化的很好描述,特别是舌上皮对侧的味蕾。实验以细胞角蛋白8抗体免疫组化染色显示,单侧鼓索神经或鼓索神经+舌神经三叉神经分支横断损伤大鼠,同侧味蕾数量及体积和细胞数接近,均低于假手术大鼠,而对侧味蕾数目不随时间发生变化,但味蕾体积和细胞数会增加。提示失神经支配后可导致同侧味蕾数量缺失及形态收缩,对侧味蕾体积增大及细胞数增加,但舌神经三叉神经分支没在保持味蕾结构的完整性中扮演角色。

关键词: 神经再生, 味觉, 细胞角蛋白, 舌上皮, 味蕾, 免疫组织化学, 三叉神经障碍

Abstract:

The morphological changes that occur in the taste buds after denervation are not well understood in rats, especially in the contralateral tongue epithelium. In this study, we investigated the time course of morphological changes in the taste buds following unilateral nerve transection. The role of the trigeminal component of the lingual nerve in maintaining the structural integrity of the taste buds was also examined. Twenty-four Sprague-Dawley rats were randomly divided into three groups: control, unilateral chorda tympani nerve transection and unilateral chorda tympani nerve transection + lingual nerve transection. Rats were allowed up to 42 days of recovery before being euthanized. The taste buds were visualized using a cytokeratin 8 antibody. Taste bud counts, volumes and taste receptor cell numbers were quantified and compared among groups. No significant difference was detected between the chorda tympani nerve transection and chorda tympani nerve transection + lingual nerve transection groups. Taste bud counts, volumes and taste receptor cell numbers on the ipsilateral side all decreased significantly compared with control. On the contralateral side, the number of taste buds remained unchanged over time, but they were larger, and taste receptor cells were more numerous postoperatively. There was no evidence for a role of the trigeminal branch of the lingual nerve in maintaining the structural integrity of the anterior taste buds.

Key words: nerve regeneration, gustation, cytokeratin, tongue epithelium, immunohistochemistry,  , taste bud, trigeminal nerve disorder, NSFC grants, neural regeneration