中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (11): 1865-1868.doi: 10.4103/1673-5374.170318

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

内源性神经营养素3:可促进残存背根神经节神经元轴突发芽

  

  • 出版日期:2015-12-07 发布日期:2015-12-07
  • 基金资助:

    上海市卫生局项目(20114351),浙江省中医药基金项目(2010ZB091),浙江省自然科学基金项目(Y2090864)

Endogenous neurotrophin-3 promotes neuronal sprouting from dorsal root ganglia

Xu-yang Wang1, 2, 3, Pei-yuan Gu1, Shi-wen Chen2, Wen-wei Gao2, Heng-li Tian2, *, Xiang-he Lu3, Wei-ming Zheng3, Qi-chuan Zhuge3, Wei-xing Hu1, *   

  1. 1 Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China
    2 Department of Neurosurgery, Shanghai Jiao Tong University Affiliated 6th People’s Hospital, Shanghai, China
    3 Department of Neurosurgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China
  • Online:2015-12-07 Published:2015-12-07
  • Contact: Wei-xing Hu, M.D., Ph.D. or Heng-li Tian, M.D., weixinghu@njmu.edu.cn or ltianhengli@163.com.
  • Supported by:

    This study was supported by grants from the Shanghai Municipal Commission of Health and Family Planning, No. 20114351, the Traditional Chinese Medicine Science Funding of Zhejiang Province of China, No. 2010ZB091 and the Natural Science Foundation of Zhejiang Province of China, No. Y2090864.

摘要:

为探索内源性神经营养素3对脊髓背根切断后远期神经末梢生长的影响。实验切除成年猫左侧背根神经节L1-5和L7-S2,保留L6背根神经节,发现神经营养素3主要表达于背根神经节大神经元和脊髓椎板II层部分神经元中。切断2个月后,猫L6背根神经节中神经营养素3阳性神经元数量和神经末梢的密度增加。而腹腔注射特异性抗体阻断内源性神经营养素3,则可使L6背根神经节神经末梢的密度减少,神经营养素3阳性神经元数量增加。表明内源性神经营养素3促进脊髓背根切断后背根神经节轴突发芽的生长,参与脊髓损伤后的重建与再生。

关键词: 神经再生, 神经营养素3, 感觉神经元, 背根神经节, 神经末梢,

Abstract:

In the present study, we investigated the role of endogenous neurotrophin-3 in nerve terminal sprouting 2 months after spinal cord dorsal root rhizotomy. The left L1–5 and L7–S2 dorsal root ganglia in adult cats were exposed and removed, preserving the L6 dorsal root ganglia. Neurotrophin- 3 was mainly expressed in large neurons in the dorsal root ganglia and in some neurons in spinal lamina II. Two months after rhizotomy, the number of neurotrophin-3-positive neurons in the spared dorsal root ganglia and the density of neurite sprouts emerging from these ganglia were increased. Intraperitoneal injection of an antibody against neurotrophin-3 decreased the density of neurite sprouts. These findings suggest that endogenous neurotrophin-3 is involved in spinal cord plasticity and regeneration, and that it promotes axonal sprouting from the dorsal root ganglia after spinal cord dorsal root rhizotomy.

Key words: nerve regeneration, neurotrophin-3, sensory neurons, dorsal root ganglion, cats, nerve terminal, neural regeneration