中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 94-100.doi: 10.4103/1673-5374.175052

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

复方中药参芪扶正液对脑缺血再灌注损伤老龄大鼠的多重神经保护

  

  • 收稿日期:2015-10-07 出版日期:2016-01-15 发布日期:2016-01-15
  • 基金资助:

    陕西省科技攻关项目(2003K10-G86),国家自然科学基金(30070731)

Neuroprotective effect of Shenqi Fuzheng injection pretreatment in aged rats with cerebral ischemia/reperfusion injury

Ying-min Cai, Yong Zhang, Peng-bo Zhang, Lu-ming Zhen, Xiao-ju Sun, Zhi-ling Wang, Ren-yan Xu, Rong-liang Xue   

  1. Department of Anesthesiology, Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China
  • Received:2015-10-07 Online:2016-01-15 Published:2016-01-15
  • Contact: Yong Zhang, 15902957876@126.com.
  • Supported by:

    This study was supported by a grant from Key Technology Research and Development Program of Shaanxi Province of China, No. 2003K10-G86; the National Natural Science Foundation of China, No. 30070731.

摘要:

中药参芪扶正注射液是从传统中药黄芪和党参的提取物,实验拟证实两药复合对脑缺血再灌注损伤的多重神经保护作用。实验设计构建了假手术组、模型组和参芪扶正注射液干预组,在连续1周对大鼠尾静脉注入40mL/kg参芪扶正注射液后建立脑缺血再灌注损伤模型。与模型组比较,发现参芪扶正注射液干预组大鼠脑组织含水量和Ca2+含量减少,脑梗死范围缩小,血清中乳酸脱氢酶、肌酸激酶及脑组织中丙二醛含量减少,脑组织中超氧化物歧化酶活性增加,脑组织超微结构明显改善,神经功能也得到改善。作者认为,该结果可以说明复方中药参芪扶正注射液对老龄大鼠脑缺血损伤有神经保护作用,其机制可能与清除氧自由基抑制脑组织Ca2+聚集等多重功效有关。

关键词: 神经再生, 脑缺血再灌注, 参芪扶正注射液, 老龄大鼠, 神经功能围, Ca2+, 氧自由基

Abstract:

Shenqi Fuzheng injection is extracted from the Chinese herbs Radix Astragali and Radix Codonopsis. The aim of the present study was to investigate the neuroprotective effects of Shenqi Fuzheng injection in cerebral ischemia and reperfusion. Aged rats (20–22 months) were divided into three groups: sham, model, and treatment. Shenqi Fuzheng injection or saline (40 mL/kg) was injected into the tail vein daily for 1 week, after which a cerebral ischemia/reperfusion injury model was established. Compared with model rats that received saline, rats in the treatment group had smaller infarct volumes, lower brain water and malondialdehyde content, lower brain Ca2+ levels, lower activities of serum lactate dehydrogenase and creatine kinase, and higher superoxide dismutase activity. In addition, the treatment group showed less damage to the brain tissue ultrastructure and better neurological function. Our findings indicate that Shenqi Fuzheng injection
exerts neuroprotective effects in aged rats with cerebral ischemia/reperfusion injury, and that the underlying mechanism relies on oxygen free radical scavenging and inhibition of brain Ca2+ accumulation.

Key words: nerve regeneration, cerebral ischemia/reperfusion, Shenqi Fuzheng injection, aged rats, neurological function, Ca2+, oxygen free radicals, NSFC grant, neural regeneration