中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (10): 1644-1652.doi: 10.4103/1673-5374.193245

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

电纺聚乳酸/聚吡咯纳米纤维支架修复损伤周围神经的优势

  

  • 收稿日期:2016-09-01 出版日期:2016-10-31 发布日期:2016-10-31
  • 基金资助:
    清华大学创新科学研究项目（20131089199）；国家重点研究发展项目（2016YFB0700802）；973项目（2012CB518106, 2014CB542201）

Preparation of polypyrrole-embedded electrospun poly(lactic acid) nanofbrous sca?olds for nerve tissue engineering

Jun-feng Zhou1, #, Yi-guo Wang2, 3, #, Liang Cheng1, Zhao Wu1, Xiao-dan Sun1, *, Jiang Peng2, 4, *   

  1. 1 Key Laboratory of Advanced Materials of Ministry of Education of China, School of Materials Science and Engineering, Tsinghua University, Beijing, China 2 Institute of Orthopedics, Chinese PLA General Hospital, Beijing, China 3 School of Medicine, Nankai University, Tianjin, China 4 Te Neural Regeneration Co-innovation Center of Jiangsu Province, Nantong, Jiangsu Province, China
  • Received:2016-09-01 Online:2016-10-31 Published:2016-10-31
  • Contact: Xiao-dan Sun or Jiang Peng, sunxiaodan@mail.tsinghua.edu.cn or pengjiang301@126.com.
  • Supported by:
    This research was fnancially supported by Tsinghua University Initiative Scientifc Research Program, No. 20131089199; the National Key Research and Development Program of China, No. 2016YFB0700802; the National Program on Key Basic Research Project of China (973 Program), No. 2012CB518106, 2014CB542201.

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摘要：

聚吡咯是一种生物相容性聚合物，具有良好的导电性。既往虽有将静电纺丝技术和聚吡咯结合的实验，但没有很好地解决聚吡咯电导下降的问题。实验应用静电纺丝技术通过将聚吡咯颗粒包裹于聚乳酸纳米纤维中，制备出新型含15%聚吡咯的聚乳酸/聚吡咯复合纳米纤维支架，具有电导率及电导稳定性良好，并拥有定向结构的特点。此种复合纳米纤维支架与许旺细胞以及人脐带间充质干细胞均具有良好的生物相容性，2种细胞沿纤维延伸方向定向生长良好。结果说明这种新型的聚乳酸/聚吡咯复合纳米纤维支架在修复周围神经损伤方面具有优势和转化为临床应用的前景。 

orcid: 0000-0002-5210-128X (Xiao-dan Sun), 0000-0003-4662-9288 (Jiang Peng)

关键词: 神经再生, 聚吡咯, 静电纺丝, 电导率, 电性能, Schwann细胞, 人脐带间充质干细胞, 神经组织工程, 纳米纤维支架

Abstract: Polypyrrole (PPy) is a biocompatible polymer with good conductivity. Studies combining PPy with electrospinning have been reported; however, the associated decrease in PPy conductivity has not yet been resolved. We embedded PPy into poly(lactic acid) (PLA) nanofbers via electrospinning and fabricated a PLA/PPy nanofbrous sca?old containing 15% PPy with sustained conductivity and aligned topography. Tere was good biocompatibility between the sca?old and human umbilical cord mesenchymal stem cells as well as Schwann cells. Additionally, the direction of cell elongation on the sca?old was parallel to the direction of fbers. Our fndings suggest that the aligned PLA/PPy nanofbrous sca?old is a promising biomaterial for peripheral nerve regeneration.

Key words: nerve regeneration, polypyrrole, electrospinning, conductivity, electrical property, Schwann cells, human umbilical cord mesenchymal stem cells, nerve tissue engineering, nanofbrous sca?olds, neural regeneration